Related Experiment Video

Updated: Aug 9, 2026

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Immunofluorescent microscopic findings in glomerulonephritis

S Tabassum1, K M Rahman, K Z Mamun

  • 1Department of Microbiology, Institute of Postgraduate Medicine & Research, Dhaka.

Bangladesh Medical Research Council Bulletin
|June 11, 1998
PubMed

Insights

Direct immunofluorescence (DIF) aids glomerulonephritis (GN) diagnosis by detecting immune deposits. DIF confirmed immune complex deposition in various GN types, proving valuable when histopathology is inconclusive.

Area of Science:

  • Nephrology
  • Immunopathology
  • Renal Pathology

Background:

  • Glomerulonephritis (GN) diagnosis relies on renal biopsy interpretation.
  • Histopathology and direct immunofluorescence (DIF) are key diagnostic tools.
  • Understanding immune complex deposition patterns is crucial for GN classification.

Purpose of the Study:

  • To evaluate the diagnostic utility of histopathology and DIF in suspected glomerulonephritis.
  • To correlate immune complex deposition patterns with specific GN subtypes.
  • To assess DIF's role in cases with inconclusive histopathological findings.

Main Methods:

  • Sixty renal biopsy specimens from clinically suspected GN cases were analyzed.
  • Histopathological examination was performed on all specimens.
  • Direct immunofluorescence (DIF) microscopy was used to detect immune deposits.

Main Results:

  • Histopathological diagnosis was achieved in 73.3% of cases.
  • DIF detected immune complex deposition in 46.7% of cases.
  • Immune deposits were present in membranous GN, SLE, RPGN, diffuse proliferative GN, mesangioproliferative GN, and focal glomerulosclerosis; absent in minimal change, chronic GN, and diabetic nephropathy.
  • DIF identified immune deposits in 3 cases (5%) where histopathology was inconclusive.

Conclusions:

  • DIF is a valuable adjunct to histopathology in diagnosing glomerulonephritis.
  • The presence and pattern of immune deposits identified by DIF aid in classifying GN.
  • DIF can provide diagnostic clues in cases where histopathology alone is insufficient.

More Related Videos

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.