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Monocyte chemotactic protein-1 in the inflammatory pseudotumour of the lung
E Pilozzi1, A Stoppacciaro, E Rendina
1Dipartimento di Medicina Sperimentale e Patologia, Università La Sapienza, Roma, Italy.
Insights
Inflammatory pseudotumours of the lung involve histiocyte accumulation. Monocyte chemotactic protein-1 (MCP-1) may drive the non-proliferating macrophage buildup in these lung lesions.
Area of Science:
- Pulmonology
- Immunology
- Pathology
Background:
- Inflammatory pseudotumours of the lung are characterized by histiocyte accumulation.
- Histiocyte accumulation can result from local cell proliferation or systemic monocyte recruitment.
- Understanding the underlying mechanisms is crucial for diagnosing and managing these lesions.
Observation:
- Two cases of inflammatory pseudotumour of the lung were analyzed.
- Cell proliferation was assessed using Ki-67 immunostaining.
- Cytokine production was evaluated via reverse transcriptase-polymerase chain reaction.
Findings:
- Lesions were primarily composed of non-proliferating macrophages (CD68+, CD14+, CD4+, mannose receptor+).
- Both cases exhibited mRNA transcripts for monocyte chemotactic protein-1 (MCP-1) and interleukin-6 (IL-6).
- One case also showed mRNA transcripts for interleukin-8 (IL-8).
Implications:
- Findings suggest MCP-1-induced accumulation of non-proliferating histiocytes is a key pathogenic event.
- This provides insights into the cellular and molecular basis of inflammatory pseudotumours.
- Further research can explore targeted therapies based on these identified pathways.
Abstract:
Inflammatory pseudotumour of the lung is a lesion mainly composed of histiocytes. Histiocyte accumulation may arise from local proliferation of migratory cells, from cytokine induced recruitment of monocytes from the systemic circulation, or both. Cell proliferation was investigated with Ki-67 immunostaining and cytokine production with reverse transcriptase-polymerase chain reaction in two cases of inflammatory pseudotumour of the lung. It was found that the two lesions were composed mainly of non-proliferating (Ki-67 non-binding) macrophages that stained positive for CD68, CD14, CD4, and mannose receptor. Both cases contained mRNA transcripts for monocyte chemotactic protein-1 (MCP-1), a monocyte chemoattractant, and for interleukin 6 (IL-6), an inducer of plasma cell differentiation. One of the two cases also contained mRNA transcripts for IL-8, a neutrophil chemoattractant. These findings are consistent with the possibility that accumulation of non-proliferating histiocytes induced by MCP-1 is one of the pathogenic events occurring in inflammatory pseudotumour of the lung.
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