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Updated: Aug 8, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Tubules of the trans Golgi apparatus visualized by immunoelectron microscopy

J Roth1, D J Taatjes

  • 1Department of Pathology, University of Zürich, Switzerland. juergen.roth@pty.usz.ch

Histochemistry and Cell Biology
|July 29, 1998
PubMed

Insights

The Golgi apparatus

Area of Science:

  • Cell Biology
  • Biochemistry

Background:

  • Tubules are key components of the Golgi apparatus, forming a dynamic network at its trans side.
  • The trans-Golgi network (TGN) is crucial for protein modification and sorting.

Purpose of the Study:

  • To investigate the structural characteristics of the TGN and its connection to the Golgi stack.
  • To identify the molecular composition and functional roles of TGN tubules and vesicles.

Main Methods:

  • Immuno-electron microscopy of Lowicryl K4M embedded human enterocytes.
  • Detection of alpha1,3 N-acetylgalactosaminyltransferase and blood group A substance.
  • Assay for CMPase activity.

Main Results:

  • Alpha1,3 N-acetylgalactosaminyltransferase and blood group A substance were found throughout the Golgi stack and TGN.
  • CMPase activity was detected in the entire TGN.
  • Two types of tubules were identified: those connecting cisternae and those forming the TGN.
  • Trans-Golgi cisternae showed continuity with the TGN and exhibited a 'peeling-off' phenomenon.
  • Non-clathrin-coated buds and vesicles containing glycoproteins were observed on trans cisternae and TGN tubules.

Conclusions:

  • The TGN is structurally integrated with the Golgi stack, featuring distinct tubular elements.
  • These structures are involved in the transport of glycoproteins with terminal N-acetylgalactosamine residues.
  • The observed buds and vesicles are implicated in constitutive exocytosis.

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