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Interferon-gamma receptor beta-chain expression and formation of alpha- and beta-chain complexes after receptor

C Ishiharu1, K Ochiai, M Kagami

  • 1Department of Pediatrics, Sakura Hospital, Toho University School of Medicine, Sakura, Japan.

International Archives of Allergy and Immunology
|October 6, 1998
PubMed

Insights

Human peripheral eosinophils express both interferon-gamma receptor (IFN-gammaR) alpha and beta chains. These chains appear to form the functional receptor complex only after IFN-gamma binding.

Area of Science:

  • Immunology
  • Cell Biology
  • Eosinophil Function

Background:

  • The functionality of the interferon-gamma receptor (IFN-gammaR) on human peripheral eosinophils remains unclear.
  • Investigating IFN-gammaR expression and subunit association is crucial for understanding eosinophil immune responses.

Purpose of the Study:

  • To determine the expression of IFN-gammaR alpha- and beta-chains on human peripheral eosinophils.
  • To investigate the association of these chains upon IFN-gamma binding.

Main Methods:

  • Flow cytometry was used to assess protein expression of IFN-gammaR alpha and beta chains on eosinophils from patients with asthma and hypereosinophilic syndrome.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect mRNA expression for both IFN-gammaR subunits.

Main Results:

  • Eosinophils from all patients tested expressed both IFN-gammaR alpha and beta chains at the protein level.
  • mRNA for both IFN-gammaR alpha and beta subunits was detected in these eosinophils.
  • Coprecipitation of the alpha and beta chains was observed exclusively after stimulation with IFN-gamma.

Conclusions:

  • Human peripheral eosinophils express both alpha and beta subunits of the IFN-gamma receptor.
  • The formation of the functional IFN-gamma receptor complex on eosinophils appears to be ligand-dependent, occurring after IFN-gamma binding.
Abstract

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