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A diagnostic in vitro urine assay for interstitial cystitis

S Keay1, C O Zhang, M K Hise

  • 1Department of Medicine, University of Maryland School of Medicine, Department of Veterans Affairs Medical Center, Baltimore 21201, USA.

Urology
|December 4, 1998
PubMed

Insights

Urine from women with interstitial cystitis (IC) significantly inhibits bladder cell growth. This antiproliferative activity in urine may help diagnose IC noninvasively.

Area of Science:

  • Urology
  • Cell Biology
  • Biochemistry

Background:

  • A previously identified urine factor inhibits normal bladder epithelial cell proliferation.
  • This factor is more prevalent in interstitial cystitis (IC) patients than in controls.

Purpose of the Study:

  • To determine if the urine antiproliferative factor is specific to IC.
  • To investigate its presence in other urogenital inflammatory conditions.

Main Methods:

  • Urine samples were collected from women with IC, bacterial cystitis, vulvovaginitis, and asymptomatic controls.
  • Bladder epithelial cell proliferation was measured using 3H-thymidine incorporation in vitro.

Main Results:

  • Urine from 86% of IC patients significantly inhibited cell proliferation.
  • Inhibition was observed in 8% of asymptomatic controls, 12% of bacterial cystitis patients, and 0% of vulvovaginitis patients (P < 0.001).
  • A cutoff of 25% inhibition achieved 91.4% sensitivity and 90.6% specificity for IC diagnosis.

Conclusions:

  • Measurement of urine antiproliferative activity shows promise as a noninvasive diagnostic tool for IC in women.
Abstract

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