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The light chain of CD98 is identified as E16/TA1 protein

B A Mannion1, T V Kolesnikova, S H Lin

  • 1Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.

The Journal of Biological Chemistry
|December 5, 1998
PubMed

Insights

The CD98 light chain, crucial for cell function, is identified as the TA1/E16 protein. This finding clarifies the CD98 protein complex composition and function.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Immunology

Background:

  • The CD98 protein complex is vital for cellular processes.
  • The precise identity of the CD98 light chain has remained unclear.

Purpose of the Study:

  • To identify the molecular identity of the CD98 light chain.
  • To confirm the relationship between CD98 light chain and TA1/E16 protein.

Main Methods:

  • Purification of the CD98 protein complex using monoclonal antibodies.
  • Peptide sequencing via mass spectrometry.
  • Immunoblotting and co-immunoprecipitation assays.
  • Transfection of cells with E16 cDNA.

Main Results:

  • Partial amino acid sequencing revealed identity to human E16 and rat TA1 proteins.
  • Antibodies against TA1/E16 specifically recognized the CD98 light chain.
  • Co-immunoprecipitation confirmed the association between CD98 heavy chain and E16.
  • Genetic reconstitution experiments validated the findings.

Conclusions:

  • The CD98 light chain is definitively identified as the TA1/E16 protein.
  • This identification is supported by sequence, antibody, genetic, and cellular data.
  • The study clarifies the composition of the functional CD98 protein complex.

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