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Interleukin 1 (IL-1) causes changes in lateral and rotational mobilities of IL-1 type I receptors

C Guo1, K E Georgiadis, S K Dower

  • 1Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301, USA.

Biochemistry
|February 4, 1999
PubMed

Insights

Interleukin-1 (IL-1) binding reduces the cell surface mobility of the IL-1 type I receptor (IL-1 RI). This interaction requires the receptor's cytoplasmic tail and links IL-1 RI to the cell's cytoskeleton, impacting signal initiation.

Area of Science:

  • Cell biology
  • Immunology
  • Molecular biology

Background:

  • The Interleukin-1 type I receptor (IL-1 RI) mediates cellular responses to IL-1.
  • Understanding IL-1 RI's cell surface dynamics is crucial for deciphering its signaling mechanisms.

Purpose of the Study:

  • To investigate the IL-1-dependent physical interactions and mobility changes of IL-1 RI on intact cells.
  • To identify the role of the IL-1 RI cytoplasmic tail in ligand-induced alterations.

Main Methods:

  • Lateral mobility was measured using fluorescence photobleaching recovery with a specific anti-IL-1 RI antibody (M5).
  • Rotational mobility was assessed via phosphorescence anisotropy decay measurements.
  • Detergent insolubility was analyzed to determine IL-1 RI association with the cytoskeleton.

Main Results:

  • IL-1 binding significantly reduced the lateral mobility of wild-type IL-1 RI but not a mutant lacking its cytoplasmic tail.
  • IL-1 decreased IL-1 RI rotational mobility and increased its association with cytoskeletal residues.
  • IL-1 receptor antagonist protein (IL-1ra) induced partial changes in IL-1 RI mobility and detergent insolubility.

Conclusions:

  • The cytoplasmic tail of IL-1 RI is essential for IL-1-induced changes in lateral mobility.
  • Ligand binding induces physical alterations in IL-1 RI, including reduced mobility and cytoskeletal association.
  • These ligand-dependent interactions may play a role in initiating signals mediated by IL-1 RI.

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