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A critical tyrosine residue in the cytoplasmic tail is important for CD1d internalization but not for its basolateral

D G Rodionov1, T W Nordeng, K Pedersen

  • 1Division of Molecular Cell Biology, Department of Biology, University of Oslo, Oslo, Norway.

Journal of Immunology (Baltimore, Md. : 1950)
|February 11, 1999
PubMed

Insights

The CD1d molecule is internalized from the cell surface and traffics to endosomes, suggesting a role in antigen presentation. Its cytoplasmic tail contains signals for both internalization and basolateral sorting.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The CD1 family of polypeptides, including CD1b and CD1d, are involved in T cell response stimulation.
  • CD1b molecules present bacterial cell wall antigens to T cells, acquiring them in endosomes.
  • The antigen presentation mechanism of CD1d remains largely uncharacterized.

Purpose of the Study:

  • To investigate the intracellular trafficking of human CD1d.
  • To identify potential cellular compartments involved in CD1d's antigen acquisition.
  • To determine the role of CD1d's cytoplasmic tail in its trafficking.

Main Methods:

  • Studied intracellular trafficking of human CD1d in Madin-Darby canine kidney (MDCK) and COS cells.
  • Analyzed CD1d localization after internalization from the plasma membrane.
  • Investigated the function of the cytoplasmic tail, including internalization and sorting signals.

Main Results:

  • CD1d was found in endosomal compartments following internalization.
  • Evidence suggests CD1d may acquire exogenous ligands in the same compartments as MHC class II and CD1b.
  • A tyrosine-based sorting signal in the CD1d cytoplasmic tail is essential for internalization.
  • A distinct signal within the cytoplasmic tail directs basolateral sorting.

Conclusions:

  • Human CD1d undergoes intracellular trafficking to endosomes, similar to CD1b.
  • CD1d likely acquires exogenous antigens within endosomal compartments.
  • Distinct signals in the CD1d cytoplasmic tail regulate both internalization and basolateral localization.

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