Video Experimental Relacionado
Updated: Jul 14, 2026
11:44
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Bases estructurales para el reconocimiento selectivo de oligosacáridos por DC-SIGN y DC-SIGNR
H Feinberg1, D A Mitchell, K Drickamer
1Department of Structural Biology, University School of Medicine, Stanford, CA 94305, USA.
Science (New York, N.Y.)
|December 12, 2001
Insights
La molécula de adhesión intracelular específica de las células dendríticas-3 agarrando la nonintegrina (DC-SIGN) se une al VIH, promoviendo la infección. Comprender la unión de DC-SIGN y DC-SIGNR a los oligosacáridos con alto contenido de manosa puede conducir a nuevos fármacos profilácticos contra el VIH.
Área de la Ciencia:
- Inmunología Inmunología.
- Virología Virología.
- Biología Estructural Biología estructural.
Sus antecedentes:
- La no integrina agarradora de la molécula de adhesión intracelular específica de células dendríticas-3 (DC-SIGN) es una lectina de tipo C en las células dendríticas que se une a ICAM-3, mediando las interacciones iniciales de las células T.
- DC-SIGN y el receptor DC-SIGNR relacionado se unen a los oligosacáridos en la envoltura del virus de la inmunodeficiencia humana (VIH), lo que mejora la infección viral de las células T.
Objetivo del estudio:
- Para aclarar la base estructural de DC-SIGN y DC-SIGNR reconocimiento de oligosacáridos.
- Explorar el potencial de estas interacciones para el desarrollo de nuevos fármacos profilácticos contra el VIH.
Principales métodos:
- Se utilizó cristalografía de rayos X para determinar las estructuras de los dominios de reconocimiento de carbohidratos de DC-SIGN y DC-SIGNR unidos a oligosacáridos.
- Se llevaron a cabo estudios vinculantes para evaluar la selectividad de estos receptores para oligosacáridos específicos.
Principales resultados:
- Las estructuras cristalinas revelaron cómo DC-SIGN y DC-SIGNR se unen a estructuras específicas de oligosacáridos.
- Los estudios vinculantes confirmaron que estos receptores reconocen selectivamente los oligosacáridos endógenos de alta manosa.
Conclusiones:
- El reconocimiento selectivo de los oligosacáridos altos en manosa por DC-SIGN y DC-SIGNR proporciona una comprensión molecular de su papel en la infección por VIH.
- Dirigirse a estas interacciones representa una nueva estrategia prometedora para desarrollar una profilaxis efectiva del VIH.
Abstracto
No abstract available in PubMed .
Videos de Conceptos Relacionados
01:44What are Carbohydrates?
Overview
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
02:21Naming Enantiomers
The naming of enantiomers employs the Cahn–Ingold–Prelog rules that involve assigning priorities to different substituent groups at a chiral center. Each enantiomer, being a distinct molecule, is assigned a unique name by the Cahn–Ingold–Prelog (CIP) rules, also called the R–S system. The prefix R- or S- attached to the chiral centers in an enantiomer is dependent on the spatial arrangement of the four substituents on the chiral center. The R–S system essentially comprises three steps:...
01:24Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
01:28Structures of Carboxylic Acid Derivatives
Structure of Carboxylic Acid Derivatives
Carboxylic acid derivatives contain an acyl group attached to a heteroatom such as chlorine, oxygen, or nitrogen. The carbonyl carbon and oxygen are both sp2-hybridized with an unhybridized p orbital.
The three sp2 orbitals of the carbonyl carbon form three σ bonds, one each with the carbonyl oxygen, the α carbon, and the heteroatom, whereas the other two sp2 orbitals of the carbonyl oxygen are occupied by the lone pairs. Further, the unhybridized p...
Carboxylic acid derivatives contain an acyl group attached to a heteroatom such as chlorine, oxygen, or nitrogen. The carbonyl carbon and oxygen are both sp2-hybridized with an unhybridized p orbital.
The three sp2 orbitals of the carbonyl carbon form three σ bonds, one each with the carbonyl oxygen, the α carbon, and the heteroatom, whereas the other two sp2 orbitals of the carbonyl oxygen are occupied by the lone pairs. Further, the unhybridized p...
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...