Video Experimental Relacionado

Updated: Jan 31, 2026

Murine Model of Leukemia Relapse to Induction Chemotherapy for Acute Lymphoblastic Leukemia

Murine Model of Leukemia Relapse to Induction Chemotherapy for Acute Lymphoblastic Leukemia

Published on: October 17, 2025

Asociación entre el inmunofenotipo leucémico y la supervivencia general en pacientes con leucemia promielocítica

Xuan Zha¹, Li Ma², Yun Yue²

¹Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Frontiers in cell and developmental biology

|January 30, 2026

Insights

La inmunofenotipificación por citometría de flujo revela que la expresión de CD2 y CD200 son factores pronósticos adversos independientes en pacientes con leucemia promielocítica aguda (LPA), lo que impacta la supervivencia general (SG). La alta expresión de estos marcadores indica un peor pronóstico para la LPA.

Área de la Ciencia:

Hematología
Oncología
Inmunología

Sus antecedentes:

La leucemia promielocítica aguda (LPA) es un subtipo distinto de leucemia mieloide aguda (LMA).
El diagnóstico preciso y la estratificación pronóstica en la LPA son fundamentales para los resultados de los pacientes.
La inmunofenotipificación por citometría de flujo (FCM) es una herramienta diagnóstica clave.

Objetivo del estudio:

Investigar la asociación entre marcadores específicos del fenotipo inmunitario y la supervivencia general (SG) en pacientes con LPA.
Identificar nuevos marcadores pronósticos para la LPA utilizando análisis de FCM.

Principales métodos:

Estudio de cohorte retrospectivo que analiza datos inmunofenotípicos de FCM de 72 pacientes con LPA.
Se utilizaron modelos de riesgos proporcionales de Cox y análisis de supervivencia de Kaplan-Meier para evaluar los factores pronósticos.
Se evaluaron marcadores específicos para su asociación con la SG, incluidos CD56, CD2, CD34 y CD200.

Principales resultados:

Las blastos de LPA mostraron perfiles inmunofenotípicos distintos en comparación con la LMA no LPA, con menor expresión de CD34, CD56, CD200.
El análisis univariante indicó que la expresión de CD34, CD2, CD56 y CD200 se asoció con una peor SG.
El análisis multivariante identificó CD2 y CD200 como factores pronósticos adversos independientes para la SG en pacientes con LPA.

Conclusiones:

Los pacientes con LPA presentan cambios inmunofenotípicos únicos en comparación con la LMA no LPA.
La expresión de CD56, CD2, CD34 y CD200 se correlaciona significativamente con la SG en la LPA.
La alta expresión pretratamiento de CD2 y CD200 puede servir como indicadores pronósticos adversos para la LPA.

Read full analysis

Abstracto

Introduction:

Acute promyelocytic leukemia (APL) is a distinct subtype of acute myeloid leukemia (AML) with unique clinical features. Flow cytometry (FCM) immunophenotyping analysis is crucial for accurate diagnosis and prognostic stratification. This study aims to explore the association between specific immune phenotype markers in APL patients and overall survival (OS).

Methods:

In this retrospective cohort study, immunophenotypic data from 72 APL patients were analyzed by FCM. Continuous and categorical variables are presented as mean ± standard deviation and frequency (percentage), respectively. Group comparisons were performed using ANOVA and Chi-square tests. Cox proportional hazards models were used to identify prognostic factors for OS, with results expressed as hazard ratios (HRs) and 95% confidence intervals (CIs). Kaplan-Meier survival analysis was employed to assess the impact of CD56, CD2, CD34, and CD200 expression on OS. Subgroup analyses were conducted based on age, gender, white blood cell count (WBC), and disseminated intravascular coagulation (DIC).

Results:

The baseline age (p = 0.513) and gender (p = 0.881) were comparable across different PML-RARα isoform groups. Compared to non-APL AML, APL blasts were characterized by significantly higher expression of CD33, CD13, CD9, and MPO (all p < 0.05), and lower expression of CD7, CD34, CD56, CD38, CD200, and HLA-DR (all p < 0.001). The PML-RARα (S-type) group showed relatively higher expression of CD34, CD2, and CD200 than the L-type group. Univariate Cox analysis revealed that expression of CD34, CD2, CD56, and CD200 were all significantly associated with poorer OS. After multivariate adjustment, CD2 (adjusted HR = 1.04, 95% CI: 1.01-1.07, p = 0.004) and CD200 (adjusted HR = 1.04, 95% CI: 1.01-1.06, p = 0.009) remained independent adverse prognostic factors. Subgroup analysis confirmed that the negative prognostic impact of CD2 and CD200 expression was consistent across different patient subgroups.

Conclusion:

Compared with non-APL-AML patients, APL patients (PML-RARα (S-type) and PML-RARα (L-type)) exhibit unique immunophenotypic changes. The expression frequencies of CD56, CD2, CD34, and CD200 in leukemia cells are significantly correlated with the OS of APL patients, and the high expression of these indicators before treatment may be an adverse prognostic factor for APL patients.

Palabras clave:

gen de fusión PML-RARα leucemia promielocítica aguda citometría de flujo inmunofenotipo supervivencia general

Más Videos Relacionados

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)

In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)

Published on: August 1, 2025

Videos de Conceptos Relacionados

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Survival Curves

Survival Curves

Survival curves are graphical representations that depict the survival experience of a population over time, offering an intuitive way to track the proportion of individuals who remain event-free at each time point. These curves are widely used in fields such as medicine, public health, and reliability engineering to visualize and compare survival probabilities across different groups or conditions.
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...

Survival Tree

Survival Tree

Survival trees are a non-parametric method used in survival analysis to model the relationship between a set of covariates and the time until an event of interest occurs, often referred to as the "time-to-event" or "survival time." This method is particularly useful when dealing with censored data, where the event has not occurred for some individuals by the end of the study period, or when the exact time of the event is unknown.
Building a Survival Tree
Constructing a...

Acute Pyelonephritis II: Diagnostic Studies and Management

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...

Association Areas of the Cortex

Association Areas of the Cortex

Association areas are regions of the cerebral cortex that do not have a specific sensory or motor function. Instead, they integrate and interpret information from various sources to enable higher cognitive processes such as memory, learning, and decision-making. Some key association areas include the following:
Prefrontal Association Area: This area is located in the frontal lobe and is involved in planning, decision-making, and moderating social behavior. It connects with primary motor areas,...

Associative Learning

Associative Learning

Associative learning is a fundamental concept in behavioral psychology, wherein a connection is established between two stimuli or events, leading to a learned response. This process is critical in understanding how behaviors are acquired and modified. Conditioning, the mechanism through which associations are formed, can be divided into two main types: classical conditioning and operant conditioning, each elucidating different aspects of associative learning.
Classical conditioning, also known...