関連する実験動画

Updated: Jul 19, 2026

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

SynCAMは,シナプス結合を駆動するシナプス結合分子です

Thomas Biederer1, Yildirim Sara, Marina Mozhayeva

  • 1Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Thomas.Biederer@UTSouthwestern.edu

Science (New York, N.Y.)
|August 31, 2002
PubMed

Insights

SynCAMは脳特異のタンパク質で,シナプス形成に不可欠な細胞粘着分子として作用します. 非ニューロン細胞でのその発現はシナプスアセンブリを誘発し,ニューロン通信における重要な役割を実証しました.

科学分野:

  • 神経科学は神経科学である.
  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.

背景:

  • シナプスは神経細胞のコミュニケーションに不可欠であり,複雑なシナプス前およびシナプス後アセンブリを含む.
  • シナプス形成の分子メカニズムを理解することは,神経科学の研究の鍵です.

研究 の 目的:

  • シナプスの形成と機能における,脳特有のタンパク質であるSynCAMの役割を調査する.
  • SynCAMがシナプスで細胞粘着分子として作用するかどうかを判断する.

主な方法:

  • 免疫グロブリン領域を含むタンパク質であるSynCAMを研究した.
  • 細胞内PDZドメインタンパク質とのSynCAMの相互作用を調べました.
  • ニューロン細胞および非ニューロン細胞におけるシナプスアセンブリに対するSynCAM発現 (全長および細胞質尾) の影響を評価した.
  • 非ニューロン細胞における再構成されたグルタマタージックシナプス伝達.

主要な成果:

  • SynCAMは,シナプスにおける同型細胞粘着分子として機能します.
  • SynCAMサイトプラズマ尾の発現は,ニューロンにおけるシナプスアセンブリを阻害した.
  • 非ニューロン細胞における全長SynCAM発現は,共培養ヒポカンパニューロンによるシナプス形成を誘発した.
  • 機能的なグルタマタージックシナプス伝達は,グルタマート受容体とSynCAMをノンニューロン細胞で共発現することによって達成されました.

結論:

  • SynCAMは,シナプスの組立と機能の重要な仲介者です.
  • 単一の粘着分子 (SynCAM) と受容体 (グルタミン酸受容体) は,機能的なポストシナプス反応に十分である可能性があります.
  • SynCAMは,脳内のシナプス接続の確立に重要な役割を果たします.
要旨

No abstract available in PubMed .

さらに関連する動画

Operation of the Collaborative Composite Manufacturing (CCM) System10:09

Operation of the Collaborative Composite Manufacturing (CCM) System

Published on: October 1, 2019

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells12:14

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

Published on: June 2, 2022

関連する概念動画

C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...