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鉴定DC-SIGN,一种新型树突细胞特异性ICAM-3受体,它支持初级免疫反应

T B Geijtenbeek1, R Torensma, S J van Vliet

  • 1Department of Tumor Immunology, University Medical Center St. Radboud, Nijmegen, The Netherlands.

Cell
|March 18, 2000
PubMed

Insights

休息的T细胞使用ICAM-3最初与树突细胞 (DC) 接触. 一种新的DC受体,DC-SIGN,与ICAM-3结合,调解T细胞粘附和增殖,这对免疫反应至关重要.

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 启动初级免疫反应需要树突细胞 (DC) 和休息T细胞之间的接触.
  • 细胞间粘附分子 (ICAMs) 在免疫系统内的细胞相互作用中起着关键作用.

研究的目的:

  • 研究ICAM-3在与DCs初次接触时对静止T细胞的作用.
  • 为了确定负责结合ICAM-3和调解T细胞粘附的特定DC受体.

主要方法:

  • 利用DC-T细胞相互作用的体外和体外模型.
  • 采用基于抗体的抑制试验来评估DC-SIGN的功能作用.
  • 研究了ICAM-3和DC-SIGN之间的结合亲和关系.

主要成果:

  • 鉴定了DC-SIGN,一种在DC上高度表达的C型乳素,作为ICAM-3的高亲和度受体.
  • 证明DC-SIGN在T细胞和DC之间调解了短暂的粘附.
  • 表明阻断DC-SIGN的抗体抑制了休息T细胞的DC诱导的增殖.

结论:

  • DC-SIGN是T细胞和DCs之间初始接触的关键调解者.
  • 对ICAM-3的DC-SIGN结合稳定了DC-T细胞结合体,促进了T细胞受体的参与和随后的T细胞激活.
  • 这些发现凸显了DC-SIGN在启动适应性免疫反应中的关键作用.
抽象的

No abstract available in PubMed .

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