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分析前条件对淋巴细胞数量的影响

Undine Proschmann1, Puya Shalchi Amirkhiz1, Pauline Andres1

  • 1Center of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, Germany.

International journal of molecular sciences
|September 9, 2023
PubMed

Insights

分析前的条件显著影响免疫细胞计数,特别是储存温度. 仔细考虑这些因素对于在临床实践中准确解释实验室结果至关重要.

科学领域:

  • 免疫学 免疫学 免疫学
  • 临床实验室科学 临床实验室科学

背景情况:

  • 淋巴细胞是多发性硬化症 (MS) 病原体的核心.
  • 针对淋巴细胞的免疫调节疗法对于MS治疗至关重要.

研究的目的:

  • 调查分析前变量如何影响免疫细胞计数.
  • 确定这些分析前效应对免疫细胞分析的临床相关性.

主要方法:

  • 分析了来自20名健康捐献者的血液样本.
  • 评估了储存温度,持续时间,门应用,身体位置和吸入力的影响.
  • 免疫细胞频率使用多色流细胞计量量化.

主要成果:

  • 在37°C保存24小时,显著降低了免疫细胞的数量.
  • 围的持续时间,身体的位置和吸气力没有显著的影响.
  • 储存温度成为影响细胞计数的分析前关键因素.

结论:

  • 分析前的条件,特别是储存温度,显然会改变免疫细胞的数量.
  • 免疫细胞亚群的实验室值需要仔细解释,考虑到分析前的变量.
  • 标准化预分析程序对于可靠的诊断和研究结果至关重要.
抽象的

No abstract available in PubMed .

关键词:
自身免疫性疾病 自身免疫性疾病流动细胞测量是指流动细胞测量.淋巴细胞 淋巴细胞在分析前的条件.储存温度 储存温度 储存温度储存时间 储存时间

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