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双功能免疫亲和磁性纳米粒子,用于基于宿主-客人相互作用的高效分离外体

Shuailong Pei1, Wanting Sun1, Qiang Han2

  • 1College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116000, PR China; MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Laboratory of Flexible Electronics Technology, Center for Synthetic and Systems Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Tsinghua University, Beijing, 100084, PR China.

Talanta
|February 21, 2024
PubMed

Insights

双功能免疫亲和磁纳米粒子 (BI-NPs) 有效地将外体从体液中隔离出来. 这种方法提供了高纯度和产量,在疾病诊断中推进了外体组应用.

科学领域:

  • 生物技术是生物技术.
  • 纳米技术 纳米技术
  • 生物化学 生物化学

背景情况:

  • 外体隔离对疾病诊断和预后至关重要.
  • 在体液中外体细胞的低度需要高效的隔离技术.

研究的目的:

  • 开发一种用于快速和低损失外体隔离的新方法.
  • 为了创建双功能免疫亲和磁性纳米粒子 (BI-NP),用于外体捕获和化.

主要方法:

  • 通过宿主-客人相互作用将Ti4+和抗体与磁性纳米粒子结合而构建的BI-NP.
  • 使用BI-NPs从细胞培养超中捕获的外体.
  • 使用α-CD和10%氨溶液化出外体.

主要成果:

  • 获得的外体蛋白质含量高达97微克/毫升.
  • 鉴定了2822个蛋白质群,其中1060个来自外体.
  • 获得的外体蛋白质质量是超离心法 (UC) 的2倍以上.
  • 由于双亲和轻度化,BI-NPs表现出高纯度的丰富性能.

结论:

  • BI-NP提供了一种高效和温和的方法来对外体隔离.
  • 这种方法提高了外体丰富的纯度和产量.
  • 在疾病诊断和预后中,BI-NP促进了外体细胞的应用.
抽象的

No abstract available in PubMed .

关键词:
双功能免疫亲和磁性纳米粒子.外基因组是外基因组的组成部分.主机与客人的互动.分离 分离 分离 分离.

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