相关实验视频

Updated: May 5, 2026

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
09:33

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

13.2K

在Fagopyrum Calli中对表观遗传修饰进行免疫化

Alicja Tomasiak1, Katarzyna Sala1, Agnieszka Braszewska2

  • 1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.

Methods in molecular biology (Clifton, N.J.)
|March 27, 2024
PubMed

Insights

本研究详细介绍了植物组织,特别是Fagopyrum calli.的免疫染色方案. 这些方法允许使用特定抗体检测基因组和DNA甲基化和乙化.

科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 免疫抑制对于可视化组织中的蛋白质定位和表达至关重要.
  • 特定抗体用于检测点蛋白质,帮助研究.
  • 基因组和DNA修改是影响基因表达的关键表观遗传标记.

研究的目的:

  • 提供一个详细的协议,用于固定Fagopyrum calli. 免疫染.
  • 为了能够检测素H3和H4的甲基化和乙化.
  • 为了促进植物组织中DNA甲基化模式的分析.

主要方法:

  • 该协议涉及固定Fagopyrum calli.的免疫染色.
  • 史蒂德曼的嵌用于组织的保存.
  • 采用了9种针对特定基因素和DNA甲基化/乙化位点的抗体.

主要成果:

  • 描述的协议允许成功地对Fagopyrum calli.进行免疫染.
  • 特定的抗体可以可视化组织组织蛋白的修饰 (甲基化,乙化).
  • 该方法有效地检测植物样本中的DNA甲基化.

结论:

  • 该协议提供了一种可靠的方法来研究Fagopyrum的表观遗传修饰.
  • 这种技术对于了解植物的基因调节有价值.
  • 详细的程序支持可重复的植物表观遗传学研究.
抽象的

No abstract available in PubMed .

关键词:
抗体是对抗体的一种.卡勒斯 (Callus) 是一个叫做的人.通过DNA甲基化.这就是Fagopyrum.基斯乙化 基斯乙化希斯甲基化 希斯甲基化免疫制 免疫制 免疫制

更多相关视频

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry07:13

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Published on: August 16, 2016

7.3K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.7K

相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
17.5K