相关实验视频

Updated: Jun 29, 2025

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

12.0K

深度学习辅助的免疫类药物数据分析

Wassim Gabriel1, Mario Picciani1, Matthew The2

  • 1Computational Mass Spectrometry, TUM School of Life Sciences, Technical University of Munich, Freising, Germany.

Methods in molecular biology (Clifton, N.J.)
|March 29, 2024
PubMed

Insights

深度学习模型增强了对人类白细胞抗原 (HLA) 的质谱分析,提高了识别精度. 这种方法有助于发现疾病特异性和新表位,克服免疫学中的计算挑战.

科学领域:

  • 质谱测量质量谱测量
  • 免疫类药物 免疫类药物
  • 计算生物学 计算生物学

背景情况:

  • 液体染色学合质谱法 (LC-MS/MS) 对于识别人类白细胞抗原 (HLA) 来说至关重要.
  • 与标准蛋白质组学相比,分析HLA具有独特的计算和统计挑战.
  • 基于碎片离子强度的得分显著改善了的识别,特别是对于非性.

研究的目的:

  • 详细说明应用深度学习模型在基于质谱的免疫学中的程序.
  • 展示如何使用最先进的深度学习工具分析和验证光谱数据.
  • 展示深度学习对HLA的识别和新表位发现的好处.

主要方法:

  • 利用像Prosit这样的深度学习框架来预测碎片离子强度和保留时间.
  • 应用诸如通用频谱探测器 (USE) 和 Oktoberfest (在线/离线) 等工具进行光谱分析.
  • 利用基于强度的评分来增强质谱数据中的匹配.

主要成果:

  • 深度学习辅助分析增加了可靠识别的HLA的数量.
  • 有助于发现确切识别的新表位物.
  • 协助评估密码性,包括拼接.

结论:

  • 深度学习模型为HLA分析中的计算挑战提供了强大的解决方案.
  • 这些方法提高了免疫学研究的准确性和范围.
  • 描述的程序提供了一个框架,通过改进的鉴定来推进个性化医疗.
抽象的

No abstract available in PubMed .

关键词:
深度学习是一种深度学习.免疫类药物 免疫类药物质谱测量质量谱测量酸标识 酸标识现在,我们可以把它转化为 Prosit Prosit.重新进行得分.视觉化 视觉化 视觉化

更多相关视频

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.0K
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.2K

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K