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冷保存对人体PBMCs细胞因子分泌和多功能性的影响:一项比较研究

Aline Linder1, Kevin Portmann1, Klaus Eyer1,2

  • 1Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.

Frontiers in immunology
|October 22, 2024
PubMed

Insights

冷保存改变了人体外围血液单核细胞 (hPBMC) 的细胞因子分泌,不同影响IL-6,IL-1β,IFN-γ和IL-8的反应. 这项研究为研究人员在免疫学研究中使用冷的hPBMCs提供了关键的见解.

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学

背景情况:

  • 人类外周血液单核细胞 (hPBMC) 对研究和临床应用至关重要.
  • 由于样本可用性的挑战,冷保存通常是必要的.
  • 之前的研究表明,冷保存对hPBMC细胞因子分泌的影响有矛盾的结果.

研究的目的:

  • 量化冷保存对单细胞细胞因子分泌和hPBMCs表达的影响.
  • 为了比较动态单细胞多重化细胞因子测量与传统的细胞内细胞因子染色.
  • 为使用冷保存的hPBMCs的研究人员提供准则.

主要方法:

  • 利用了一个平台来动态测量单细胞多重化细胞因子分泌.
  • 将结果与传统的细胞内细胞因子染色方法进行比较.
  • 刺激hPBMCs与LPS或抗CD3/CD28抗体长达72小时.

主要成果:

  • 冷保存导致IL-6,IL-1β和IFN-γ的分泌量减少,对IL-8分泌有显著影响.
  • 在冷保存后观察到TNF-α表达 (增加) 和分泌 (稳定) 之间的断开.
  • 冷保存改变了单细胞的多功能性,并以不同的方式影响了淋巴细胞和单细胞.

结论:

  • 冷保存诱导hPBMC细胞因子分泌概况的明显,但一致的变化.
  • 研究结果提供了对冷保存和刺激后免疫细胞行为的定量见解.
  • 为解释冷保存的hPBMC研究数据提供了有价值的指导.
抽象的

No abstract available in PubMed .

关键词:
冷保存的方法细胞因子分泌 细胞因子分泌人类外周血液单核细胞.多功能性的多功能性.一个单细胞分析.

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