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对流量细胞计交叉匹配测试的实验室间变量的评估:从熟练度调查中吸取的教训

Mary Carmelle Philogene1, Olga A Timofeeva2, Idoia Gimferrer3

  • 1Histocompatibility and Immunogenetics Laboratory, Virginia Commonwealth University, Richmond, VA, United States.

Human immunology
|December 3, 2024
PubMed

Insights

组织相容性实验室在检测人类白细胞抗原 (HLA) 抗体时使用流细胞计交叉匹配 (FCXM) 和抗体测试显示出良好的协议. 这种一致性对于评估移植中的免疫风险至关重要.

科学领域:

  • 免疫学和移植科学 免疫学和移植科学
  • 临床实验室医学 临床实验室医学

背景情况:

  • 精确检测人类白细胞抗原 (HLA) 抗体对于基因组相容性实验室至关重要.
  • 熟练测试调查评估HLA抗体和交叉匹配试验的实验室间一致性.

研究的目的:

  • 评估流动细胞计交叉匹配 (FCXM) 和抗体检测中的实验室间变异性.
  • 评估HLA抗体检测和交叉匹配结果在六年时间内的一致性.

主要方法:

  • 分析ASHI熟练测试 (PT) 抗体和交叉匹配 (AC) 调查结果,从6年的时间.
  • 来自国家和国际实验室的10,000多个T细胞和10,000个B细胞FCXM结果的审查.
  • 基于对FCXM的80%门和对捐赠者特异性HLA抗体 (DSA) 的90%门的共识评估.

主要成果:

  • 获得了高的共识率:T细胞FCXM的92.5%,B细胞FCXM的91.7%.
  • 达成共识的结果与供体特异性HLA抗体 (DSA) 的存在或不存在相关.
  • 7.5% (T细胞) 和8.3% (B细胞) FCXM结果的差异与DSA共识问题有关.

结论:

  • 对于FCXM试验,实验室间存在良好的共识,尽管试验方案存在差异.
  • FCXM与基于珠子的抗体检测试验有很强的相关性.
  • 结合使用FCXM和抗体测定,为免疫风险评估提供可靠的数据.
抽象的

No abstract available in PubMed .

关键词:
流量细胞计交叉匹配 流量细胞计交叉匹配进行HLA抗体测试.能力测试 能力测试 能力测试虚拟交叉匹配可以实现.

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