相关实验视频

Updated: May 23, 2025

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

7.1K

瘤微环境的细胞形态测量及其与介质素-4的免疫表达的关联

Caio Rodrigues Maia1, Maria Sara Maia de Queiroz2, Carlos Augusto Galvão Barboza2

  • 1Student of the Postgraduate Program in Dental Sciences at Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.

Tissue & cell
|March 9, 2025
PubMed

Insights

状细胞癌 (SCC) 的更深入的入侵与细胞形态的改变和减少的互白素-4 (IL-4) 表达相关. 这表明细胞变化与口腔癌中分化细胞表型的丧失之间存在潜在的联系.

科学领域:

  • 口腔病理学 口腔病理学
  • 在瘤学瘤学.
  • 细胞生物学 细胞生物学

背景情况:

  • 状细胞癌 (SCC) 是一种普遍存在的口腔恶性瘤.
  • 了解下唇SCC (LLSCC) 和口舌SCC (OTSCC) 的细胞和分子变化对于预后至关重要.

研究的目的:

  • 为了进行LLSCC和OTSCC的细胞形态分析.
  • 为了比较LLSCC和OTSCC之间的互白素-4 (IL-4) 的免疫表达.
  • 研究细胞形态,IL-4表达和瘤侵袭深度之间的关系.

主要方法:

  • 对60个SCC案件 (30个LLSCC,30个OTSCC) 的分析.
  • 每例16个恶性角质细胞的细胞形态测量.
  • 使用图像分析软件,对外膜和侧膜 (表面和深层) 进行IL-4免疫表达分析.
  • 使用ANOVA和斯皮尔曼相关性进行统计分析.

主要成果:

  • 总IL-4免疫表达在LLSCC与OTSCC相比显著更高 (p:0.007).
  • 恶性角质细胞周边/面积和入侵深度之间观察到正相关性 (p:0.009,p:0.038).
  • 在IL-4表达和核/细胞形态参数与入侵深度之间发现了负相关性 (p:0.007,p:0.008).

结论:

  • 更深入的侵入性SCC表现出更显著的核和细胞形态变化.
  • 在更深入的侵入性SCC中IL-4表达的减少可能表明分化细胞表型的丧失.
  • 形态测量和IL-4免疫表达分析为SCC进展提供了洞察力.
抽象的

No abstract available in PubMed .

关键词:
细胞形态学 细胞形态学IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-4-IL-IL-4-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL-IL is is is is is is is what you are is what you are is what you are免疫组织化学 免疫组织化学核形态测量 核形态测量 核形态测量口腔状细胞癌的癌症.

更多相关视频

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model07:49

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

20.0K
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

9.5K

相关概念视频

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K