相关实验视频

Updated: Jan 18, 2026

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
11:40

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Published on: January 30, 2018

13.6K

在促炎环境下,Caco-2肠细胞样单层对胰岛素的形态功能反应

Aurora Mazzei1, Marina Damato2, Ilenia Iaia3

  • 1Laboratory of Applied Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy.

Cells
|September 13, 2025
PubMed

Insights

胰岛素 (INS) 影响肠上皮细胞的结构和功能. 它的作用取决于炎症信号,如介质蛋白1β (IL-1β) 和干扰素 (IFN-γ),影响细胞骨组织和细胞反应.

科学领域:

  • 细胞生物学 细胞生物学
  • 胃肠病学 胃肠病学
  • 内分泌学 在内分泌学.

背景情况:

  • 胰岛素 (INS) 调节营养代谢,并影响细胞形态,包括细胞骨活动.
  • 胰岛素敏感性会影响肠道上皮细胞,影响紧密的结点和屏障透性.
  • 胰岛素在胃肠表皮质中的生理作用需要进一步阐明.

研究的目的:

  • 在炎症条件下 (IL-1β和IFN-γ) 研究胰岛素对差异化Caco-2肠细胞单层的影响.
  • 为了识别胰岛素和特定的炎症介质对肠道上皮质的形态功能变化.
  • 了解胰岛素信号如何与炎症途径相互作用以调节肠道细胞结构和功能.

主要方法:

  • 培养分化的Caco-2细胞单层.
  • 具有促炎性细胞因子的被挑战的单层:互白素1β (IL-1β) 和干扰素玛 (IFN-γ).
  • 评估胰岛素对行为细胞骨组织和相关蛋白质 (FAK,ITGB1) 和MAPK信号通路的表达的影响.

主要成果:

  • 胰岛素显著改变了Caco-2细胞中的actin细胞骨架组织.
  • 促炎性刺激 (IL-1β,IFN-γ) 调节了胰岛素对细胞形态的影响.
  • IFN-γ和胰岛素的协同作用改变了细胞骨蛋白的表达 (FAK,ITGB1) 并影响了MAPK信号传递.

结论:

  • 肠上皮细胞表现出对胰岛素的模块化反应,受不同炎症媒介的影响.
  • 胰岛素信号在肠道上皮细胞的形态功能重塑中发挥作用.
  • 在调节肠道上皮细胞行为时,胰岛素信号和炎症反应之间存在显著的相互作用.
抽象的

No abstract available in PubMed .

关键词:
在Caco-2单层中,这是一种炎症炎症炎症炎症.胰岛素信号通路是指胰岛素的信号通路.肠道上皮质 肠道上皮质

更多相关视频

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

26.7K
Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells05:51

Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells

Published on: June 15, 2013

13.4K

相关概念视频

Insulin: The Receptor and Signaling Pathways01:28

Insulin: The Receptor and Signaling Pathways

Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
2.8K
Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
6.4K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
2.2K