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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
Published on: September 29, 2012
La activación de la transcripción ligando-dependiente por los receptores nucleares requiere el complejo DRIP
C Rachez1, B D Lemon, Z Suldan
1Cell Biology Program, Memorial Sloan-Kettering Cancer Center, Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA.
Nature
|May 11, 1999
Resumen
Los investigadores identificaron trece proteínas, llamadas DRIP, que forman un complejo esencial para la transcripción génica del receptor de vitamina D (VDR). Este complejo DRIP es crucial para la activación de genes dependientes de las hormonas, destacando los cofactores compartidos entre diferentes activadores de la transcripción.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- Endocrinología Endocrinología.
Sus antecedentes:
- Los receptores nucleares regulan la transcripción génica en respuesta a los ligandos lipofílicos.
- La unión de ligandos induce cambios conformacionales, lo que permite las interacciones receptor-cofactor para la transactivación.
- Un complejo DRIP previamente identificado interactúa con el receptor de vitamina D (VDR).
Objetivo del estudio:
- Para identificar los componentes del complejo del cofactor DRIP.
- Para aclarar la función del complejo DRIP en la transactivación dependiente de las hormonas.
- Investigar la relación entre los DRIP y otros complejos de cofactores.
Principales métodos:
- Identificación y caracterización de proteínas.
- Ensayos de transcripción in vitro utilizando sistemas libres de células.
- Pruebas de transcripción basadas en cromatina.
Principales resultados:
- Se identificaron trece DRIP, que constituyen un nuevo complejo de cofactores.
- El complejo DRIP juega un papel central en la transactivación dependiente de la hormona mediada por el VDR en la cromatina.
- Las subunidades DRIP/ARC se comparten con otros complejos de cofactores (CRSP, NAT, SMCC, Mediator).
Conclusiones:
- El complejo DRIP es crítico para la transcripción génica mediada por VDR.
- Los ligandos de los receptores nucleares pueden funcionar reclutando complejos de cofactores como los DRIP.
- Distintos activadores de la transcripción pueden utilizar complejos de cofactores compartidos, lo que indica mecanismos reguladores conservados.
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