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Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Published on: September 20, 2014
Citónemos: procesos celulares que se proyectan hacia el principal centro de señalización en los discos imaginarios de
F A Ramírez-Weber1, T B Kornberg
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA.
Cell
|June 15, 1999
Resumen
Las células del disco de ala de Drosophila utilizan extensiones delgadas basadas en actina llamadas citonemas para comunicarse con los centros de señalización. Estas proyecciones, también observadas en células de ratón, sugieren un papel para los citonemas en la comunicación celular de largo alcance.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El desarrollo del disco imaginario del ala en Drosophila se basa en la señalización de la frontera del compartimento anterior / posterior.
- Comprender los mecanismos de comunicación célula-célula es crucial para los procesos de desarrollo.
Objetivo del estudio:
- Para investigar el papel de las extensiones celulares en la transmisión de señales durante el desarrollo del disco del ala.
- Para determinar si estas extensiones se conservan en todas las especies.
Principales métodos:
- Cultivo de células del disco imaginario del ala de Drosophila y células de brote de extremidades de ratón.
- Observación de las proyecciones celulares (citonemas) mediante el uso de la microscopía.
- Inducir la formación de citonemas con coculturas celulares específicas y factores de crecimiento.
Principales resultados:
- Las células del disco de las alas de Drosophila forman extensiones delgadas basadas en actina (citonemas) que se proyectan hacia el centro de señalización anterior/posterior.
- La formación de citonemas puede ser inducida por la proximidad a las células de señalización o al factor de crecimiento de los fibroblastos.
- Se observaron proyecciones similares en células de capullo de extremidades de ratón durante el cultivo.
Conclusiones:
- Los citonemas son una estructura celular conservada involucrada en la comunicación célula-célula.
- Estas proyecciones pueden mediar la señalización de largo alcance esencial para los procesos de desarrollo tanto en invertebrados como en vertebrados.
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