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Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Xyloglucan fucosyltransferase, una enzima involucrada en la biosíntesis de la pared celular de las plantas
R M Perrin1, A E DeRocher, M Bar-Peled
1Michigan State University-Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Science (New York, N.Y.)
|June 18, 1999
Resumen
Los investigadores identificaron una enzima clave en las paredes celulares de las plantas. Esta fucosiltransferasa (FTasa) agrega azúcares esenciales al xiloglucano (XG), lo que afecta el desarrollo y la defensa de las plantas.
Área de la Ciencia:
- Biología Vegetal Biología Vegetal
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Las paredes celulares de las plantas son vitales para la estructura, el crecimiento y la defensa de las plantas.
- El xiloglucano (XG) es un componente estructural primario en las paredes celulares de las dicotas, caracterizado por un residuo de fucosilo terminal.
- Las enzimas fucosiltransferasa (FTasa) son las responsables de añadir este residuo crucial de fucosa.
Objetivo del estudio:
- Identificar y caracterizar la fucosiltransferasa (FTasa) específica responsable de la modificación del xiloglucano (XG) en las plantas.
- Para clonar el gen que codifica la FTasa identificada de la Arabidopsis.
Principales métodos:
- Purificación de una FTasa de 60 kilodaltones de epicotilos de guisante.
- Secuenciación de péptidos de la FTase de guisante purificado.
- Clonado del gen homólogo de la Arabidopsis FT1.
- Generación de anticuerpos contra la Arabidopsis FTase. recombinante.
- Los ensayos de actividad enzimática utilizan proteínas de membrana de Arabidopsis solubilizadas y células COS que expresan AtFT1.1.
Principales resultados:
- Se purificó una FTasa de 60 kilodaltones de los epicotilos del guisante.
- Los datos de la secuencia de péptidos permitieron la clonación del gen homólogo de la Arabidopsis, AtFT1.1.
- Los anticuerpos confirmaron que AtFT1 codifica una FTase que modifica XG.
- La expresión de AtFT1 en células de mamíferos confería actividad de la XG FTase.
Conclusiones:
- El gen AtFT1 codifica una fucosiltransferasa de xiloglucano funcional (XG FTase).
- Esta enzima juega un papel crítico en la biosíntesis de la pared celular de las plantas.
- Comprender la función de la XG FTase proporciona información sobre el desarrollo de las plantas y los mecanismos de resistencia a las enfermedades.
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