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Enzima convertidora del factor de necrosis tumoral alfa y el factor de necrosis tumoral alfa en la cardiomiopatía
M Satoh1, M Nakamura, H Saitoh
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Iwate, Japan. staohm@iwate-med.ac.jp
Circulation
|June 29, 1999
Resumen
El aumento de la expresión de la enzima convertidora de factor de necrosis tumoral alfa (TACE) está relacionado con niveles más altos de factor de necrosis tumoral alfa (TNF-alfa) en la cardiomiopatía dilatada (DCM). Esta asociación se correlaciona con los indicadores clínicos más graves de DCM.
Área de la Ciencia:
- Cardiología Cardiología.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El factor de necrosis tumoral alfa (TNF-alfa) está implicado en la patogénesis de la cardiomiopatía dilatada (DCM).
- La enzima de conversión de TNF-alfa (TACE) procesa el precursor TNF-alfa en su forma madura.
- El papel de TACE en la producción de TNF-alfa miocárdico en DCM no se entiende completamente.
Objetivo del estudio:
- Para investigar la expresión de TACE y TNF-alfa en el miocardio de pacientes con DCM.
- Determinar la relación entre los niveles de TACE y TNF-alfa y la gravedad clínica de DCM.
Principales métodos:
- Muestras de tejido endomiocárdico de 30 pacientes con DCM y 5 controles.
- Reacción en cadena reversa transcriptasa-polimerasa cuantitativa en tiempo real para los niveles de ARNm.
- Análisis inmunohistoquímico para la expresión de proteínas.
Principales resultados:
- Tanto el TNF-alfa como el ARNm TACE se elevaron significativamente en los pacientes con DCM en comparación con los controles.
- Se observó una fuerte correlación positiva entre los niveles de TNF-alfa y TACE mRNA (r=0.779).
- Aumento de la expresión de TACE y TNF-alfa correlacionado con el diámetro sistólico final del ventrículo izquierdo superior y la fracción de eyección del ventrículo izquierdo inferior.
Conclusiones:
- La expresión elevada de TACE en el miocardio se asocia con un aumento de la expresión de TNF-alfa en el miocardio en DCM.
- Esta asociación es evidente tanto a nivel de ARNm como de proteínas.
- Los hallazgos sugieren que TACE juega un papel en la progresión de la DCM clínicamente avanzada.
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