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Videos de Conceptos Relacionados

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

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Video Experimental Relacionado

Updated: Jul 12, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
10:36

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

Estabilización de la estructura de la cromatina por PRC1, un complejo de policombos.

Z Shao1, F Raible, R Mollaaghababa

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.

Cell
|July 21, 1999
PubMed
Resumen

El complejo represor de policombos 1 (PRC1) mantiene la represión génica y compite con los complejos de remodelación SWI/SNF. PRC1 funciona independientemente de las colas de histona, lo que sugiere un mecanismo distinto en la regulación epigenética.

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A Method to Study de novo Formation of Chromatin Domains

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Last Updated: Jul 12, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
10:36

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

A Method to Study de novo Formation of Chromatin Domains
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A Method to Study de novo Formation of Chromatin Domains

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Área de la Ciencia:

  • Biología del desarrollo Biología del desarrollo.
  • La epigenética es la epigenética.
  • Biología Molecular Biología Molecular

Sus antecedentes:

  • Los genes del grupo policómbico (PcG) son cruciales para mantener la represión génica homeótica durante el desarrollo.
  • Las mutaciones de la familia SWI/SNF pueden suprimir los defectos causados por mutaciones en el gen PcG.

Objetivo del estudio:

  • Para purificar y caracterizar el complejo represivo 1 (PRC1) del Policombo.
  • Para investigar la relación funcional entre los complejos de remodelación PRC1 y SWI/SNF.

Principales métodos:

  • Purificación del complejo PRC1.
  • Ensayos para probar el efecto de PRC1 en la remodelación de matrices nucleosómicas mediadas por SWI/SNF.
  • Experimentos utilizando matrices nucleosómicas con y sin colas de histona.

Principales resultados:

  • PRC1 fue purificado y se encontró que contenía productos de varios genes PcG.
  • La preincubación de PRC1 con matrices nucleosómicas inhibió la remodelación SWI/SNF.
  • La actividad de remodelación de PRC1 se observó en matrices nucleosómicas que carecían de colas de histona.

Conclusiones:

  • PRC1 y SWI/SNF pueden competir por la plantilla nucleosómica.
  • La actividad de PRC1 en las histonas sin cola sugiere un mecanismo independiente de las interacciones de la cola de la histona, diferenciándola de otros complejos represivos como las desacetilasas.