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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
Identificación molecular de un canal catiónico eucariota no selectivo activado por estiramiento
M Kanzaki1, M Nagasawa, I Kojima
1Laboratory of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8510, Japan.
Science (New York, N.Y.)
|August 7, 1999
Resumen
Los investigadores identificaron el producto del gen MID1 de la levadura (Mid1) como un canal catiónico activado por estiramiento. Este descubrimiento proporciona el primer gen eucariota que codifica un canal que responde a los estímulos mecánicos, cruciales para las respuestas celulares.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La biofísica es la biofísica.
Sus antecedentes:
- Los canales catiónicos activados por estiramiento (SA Cat) son vitales para las respuestas celulares a los estímulos mecánicos.
- Los genes que codifican estos canales eucarióticos se mantuvieron elusivos.
- Se sabe que el producto del gen MID1 de la levadura (Mid1) es esencial para la afluencia de calcio en Saccharomyces cerevisiae.
Objetivo del estudio:
- Identificar el gen que codifica los canales catiónicos no selectivos activados por el estiramiento de los eucariotas.
- Caracterizar las propiedades funcionales de la proteína Mid1 de la levadura en la mediación de las respuestas celulares al estrés mecánico.
Principales métodos:
- Expresión funcional del gen MID1 de la levadura en las células del ovario del hámster chino (CHO).
- Medición de la conductividad del calcio y de la concentración citosólica de calcio libre bajo tensión mecánica.
- Aplicación de gadolinio, un bloqueador conocido de los canales SA Cat.
- Análisis de lámparas de parche de un solo canal.
Principales resultados:
- La expresión funcional de Mid1 en las células CHO confirió sensibilidad a la tensión mecánica.
- El estrés mecánico condujo a un aumento de la conductividad del calcio y al calcio libre citosólico.
- Estos aumentos fueron dependientes del calcio extracelular e inhibidos por el gadolinio.
- El análisis de un solo canal confirmó las funciones de Mid1 como un canal activado por estiramiento selectivo por catión, permeable al calcio.
Conclusiones:
- El producto del gen MID1 de la levadura (Mid1) funciona como un canal catiónico activado por estiramiento.
- Mid1 representa el primer gen eucariota identificado que codifica un canal catiónico activado por estiramiento.
- Este hallazgo proporciona una base molecular para la comprensión de la mecanotransducción celular en eucariotas.
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