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Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Published on: July 7, 2010
Regulación coordinada de RAG1 y RAG2 por elementos de ADN específicos del tipo de célula 5' de RAG2
W Yu1, Z Misulovin, H Suh
1Laboratory of Molecular Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Science (New York, N.Y.)
|August 14, 1999
Resumen
El estudio investigó la regulación de los genes RAG1 y RAG2, cruciales para el desarrollo de los linfocitos. Diferentes elementos genéticos que controlan su expresión sugieren un mecanismo para su integración en la evolución de los vertebrados.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Las proteínas RAG1 y RAG2 son esenciales para la recombinación V(D) J, un proceso crítico para la inmunidad adaptativa.
- Se supone que estos genes se originan de un elemento genético móvil integrado en el genoma de los vertebrados hace millones de años.
Objetivo del estudio:
- Para investigar la regulación in vivo de la expresión génica RAG1 y RAG2.
- Identificar los elementos genéticos que controlan la expresión coordinada de RAG1 y RAG2 en los linfocitos en desarrollo.
Principales métodos:
- Utilizó transgenes de cromosomas artificiales bacterianos (BAC) con indicadores fluorescentes para rastrear la expresión génica in vivo.
- Analizó las regiones reguladoras aguas arriba del gen RAG2 para identificar elementos de acción cis.
Principales resultados:
- Expresión coordinada demostrada de RAG1 y RAG2 en los linfocitos B y T.
- Se identificaron distintos elementos genéticos ubicados en el lado 5' del gen RAG2 que regulan esta expresión de coordenadas.
Conclusiones:
- Sugiere que los elementos de ADN cis asimetricamente posicionados controlan la expresión del transposón ancestral.
- Propone un mecanismo por el cual estos elementos reguladores facilitaron la captura y evolución de genes RAG en vertebrados.
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