Video Experimental Relacionado
Updated: Jul 8, 2026
11:27
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Published on: November 2, 2018
NF-kappaB es un objetivo de AKT en la señalización anti-apoptótica del PDGF
1Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, 27599-7280, USA.
Nature
|September 15, 1999
Resumen
El factor de crecimiento derivado de plaquetas (PDGF) promueve la supervivencia celular al inhibir la apoptosis. Este estudio revela que el PDGF activa el NF-kappaB a través de la vía Ras/PI(3)K/Akt/IKK, vinculando las señales de supervivencia con el control de la proliferación celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Investigación del cáncer Investigación del cáncer.
Sus antecedentes:
- La proliferación celular y la transformación están estrechamente ligadas a la apoptosis, la vía programada de la muerte celular.
- El factor de crecimiento derivado de plaquetas (PDGF) es un factor clave de supervivencia que promueve la proliferación celular, pero sus mecanismos antiapoptóticos no se han aclarado completamente.
- Comprender cómo las señales de supervivencia regulan el destino celular es crucial para la investigación del cáncer.
Objetivo del estudio:
- Investigar el papel del factor de transcripción NF-kappaB en la señalización del PDGF.
- Para dilucidar la vía molecular que media los efectos anti-apoptóticos del PDGF.
- Para vincular la señalización del factor de crecimiento con la maquinaria de transcripción que controla el destino celular.
Principales métodos:
- Se ha investigado la activación inducida por PDGF de NF-kappaB.
- Utilizó Ras, fosfatidilinositol-3-cinasa (PI(3) K), PKB/Akt e IkappaB quinasa (IKK) como componentes clave en la vía de señalización.
- Se examinó in vivo la asociación de Akt con IKK tras la estimulación del PDGF.
Principales resultados:
- Demostró el papel crítico de NF-kappaB en la señalización del PDGF.
- Identificó NF-kappaB como mediador tanto de la proliferación (a través de c-myc) como de las señales antiapoptóticas.
- Se demostró que el PDGF activa el NF-kappaB a través de una vía Ras/PI(3)K/Akt/IKK.
- Se confirmó la asociación in vivo transitorio de Akt con y la activación de IKK después de la estimulación de PDGF.
Conclusiones:
- Se ha establecido una nueva vía de señalización anti-apoptótica: Ras/PI(3)K/Akt/IKK/NF-kappaB.
- Las señales de supervivencia mediadas por PDGF vinculadas directamente a la maquinaria de transcripción.
- Proporcionó nuevos conocimientos sobre los mecanismos moleculares que rigen la proliferación celular, la supervivencia y la inhibición de la apoptosis.
Más Videos Relacionados
10:57NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
07:15Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
Videos de Conceptos Relacionados
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:22Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
02:26NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...