Video Experimental Relacionado
Updated: Jul 18, 2026
08:14
Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
Published on: August 26, 2014
La expresión del factor de transcripción deltaFosB en el cerebro controla la sensibilidad a la cocaína
M B Kelz1, J Chen, W A Carlezon
1Laboratory of Molecular Psychiatry and Yale Center for Genes and Behavior, Yale University School of Medicine, New Haven, Connecticut 06508, USA.
Nature
|September 28, 1999
Resumen
La exposición crónica a la cocaína aumenta persistentemente el deltaFosB en el cerebro.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología Molecular Biología Molecular
- Investigación de adicciones Investigación de adicciones.
Sus antecedentes:
- La exposición aguda a la cocaína induce transitoriamente las proteínas Fos en el núcleo accumbens.
- La exposición crónica a la cocaína conduce a una expresión persistente de deltaFosB en el núcleo accumbens.
- deltaFosB está implicado en la plasticidad neural asociada con el uso crónico de drogas.
Objetivo del estudio:
- Para investigar el papel del deltaFosB en la mediación de los efectos de la exposición crónica a la cocaína.
- Para determinar si la inducción del deltaFosB aumenta la sensibilidad a los efectos gratificantes y locomotores de la cocaína.
Principales métodos:
- Se utilizaron ratones transgénicos para inducir la expresión del deltaFosB en neuronas específicas del núcleo accumbens.
- La cocaína administrada para evaluar las respuestas conductuales y los cambios moleculares.
Principales resultados:
- La expresión inducida del deltaFosB aumentó la capacidad de respuesta a los efectos gratificantes y locomotores de la cocaína.
- Los efectos mediados por deltaFosB se debieron en parte al aumento de la expresión de la subunidad GluR2 del receptor de glutamato de AMPA.
- La acumulación sostenida de deltaFosB en el núcleo accumbens se correlaciona con cambios persistentes en el comportamiento.
Conclusiones:
- deltaFosB juega un papel crítico en la mejora de la sensibilidad a la cocaína.
- La expresión genética alterada por deltaFosB puede contribuir al desarrollo de la adicción a la cocaína.
- Este factor de transcripción es un objetivo terapéutico potencial para la adicción.
Más Videos Relacionados
05:44The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
Published on: April 25, 2016
08:37Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
Published on: August 27, 2016
Videos de Conceptos Relacionados
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
02:33Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...