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La evolución viral revelada por el bacteriófago PRD1 y las estructuras proteicas de la capa del adenovirus humano
S D Benson1, J K Bamford, D H Bamford
1The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Cell
|September 28, 1999
Resumen
El bacteriófago PRD1
Área de la Ciencia:
- Biología estructural Biología estructural.
- Virología Virología.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- El bacteriófago PRD1 posee una membrana interna única dentro de su caparazón proteico icosaédrico.
- La proteína principal de la capa (P3) de PRD1 es crucial para la estructura y la estabilidad de la cápside.
Objetivo del estudio:
- Para determinar la estructura cristalina de alta resolución de la proteína de capa mayor PRD1 (P3).
- Investigar las similitudes estructurales entre PRD1 y el adenovirus humano, a pesar de infectar diferentes huéspedes.
Principales métodos:
- Cristalografía de rayos X con una resolución de 1,85 A.
- Análisis estructural comparativo de las proteínas de la capa viral y la arquitectura de la cápside.
Principales resultados:
- La estructura cristalina de P3 reveló una molécula trímera con motivos de rollo de gelatina viral de ocho hebras.
- P3 comparte una homología estructural significativa con el hexón, la principal proteína de la capa del adenovirus humano.
- PRD1 y el adenovirus exhiben arquitecturas generales similares, incluidas las redes de cápsides y las proteínas de vértices.
Conclusiones:
- Las sorprendentes similitudes estructurales sugieren un origen evolutivo compartido entre el bacteriófago PRD1 y el adenovirus humano.
- Este hallazgo proporciona información sobre la evolución de los virus de ADN de doble cadena (dsDNA) en diferentes reinos huéspedes.
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