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Evidencia de que un oscilador de libre funcionamiento impulsa los eventos G1 en el ciclo celular de la levadura en
1Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 94303, USA.
Nature
|October 12, 1999
Resumen
La levadura en ciernes posee un oscilador del ciclo celular independiente de las cinasas dependientes de la ciclina, lo que permite que los eventos G1 continúen incluso cuando la división celular está bloqueada. Este oscilador asegura la progresión oportuna, similar a las células embrionarias tempranas.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Las células somáticas dependen de la finalización de eventos secuenciales para la progresión del ciclo celular.
- Las células embrionarias tempranas a menudo exhiben osciladores independientes del ciclo celular, desacoplando el tiempo del evento de la finalización.
Objetivo del estudio:
- Para investigar la presencia y las características de un oscilador del ciclo celular independiente en Saccharomyces cerevisiae.
- Para determinar si este oscilador funciona independientemente de los reguladores clave del ciclo celular como las cinasas dependientes de la ciclina.
Principales métodos:
- Se observó la activación periódica de los eventos de la fase G1 en las células de levadura que carecen de las actividades esenciales de la cinasa mitotica dependiente de la ciclina.
- Se analizaron las oscilaciones del ciclo celular en las células que responden a la feromona de apareamiento sin G1 o la actividad de la cinasa asociada a la ciclina mitótica.
- Se evaluó el papel de la actividad de la quinasa asociada a Clb en la aplicación de las dependencias del ciclo celular.
Principales resultados:
- Los eventos periódicos de G1 ocurrieron según lo programado en ausencia de fase S o mitosis, con un período similar al de las células de tipo salvaje.
- Se observaron oscilaciones similares independientemente de la actividad de la quinasa asociada a la ciclina G1 (Cln) y a la ciclina mitótica (Clb).
- Se encontró que la actividad de la quinasa asociada a Clb es esencial para prevenir eventos prematuros de G1 durante la progresión retrasada del ciclo celular.
Conclusiones:
- La levadura en ciernes Saccharomyces cerevisiae posee un oscilador del ciclo celular independiente.
- Este oscilador puede funcionar independientemente de la dinámica de la cinasa dependiente de la ciclina.
- La actividad de la cinasa dependiente de la ciclina es crucial para hacer cumplir las dependencias del ciclo celular y prevenir eventos prematuros.
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