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La colección de genes de mamíferos.
R L Strausberg1, E A Feingold, R D Klausner
1National Cancer Institute, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Science (New York, N.Y.)
|October 16, 1999
Resumen
El proyecto Colección de genes de mamíferos (MGC, por sus siglas en inglés) crea recursos de ADN complementario (ADNc) de longitud completa y accesibles al público para los investigadores. Esta iniciativa tiene como objetivo proporcionar un conjunto completo de secuencias genéticas de mamíferos y clones para el avance científico.
Área de la Ciencia:
- La genómica es la genómica.
- Biología Molecular Biología Molecular
- Biotecnología La biotecnología es la biotecnología.
Sus antecedentes:
- La necesidad de recursos completos de ADN complementario de longitud completa (ADNc) es fundamental para comprender la función génica de los mamíferos.
- Los recursos existentes pueden ser incompletos o carecer de marcos de lectura abiertos de longitud completa (ORF).
Objetivo del estudio:
- Para establecer el proyecto de Colección de genes de mamíferos (MGC).
- Generar y proporcionar acceso público a un conjunto completo de secuencias y clones ORF de longitud completa de humanos y otros mamíferos.
- Apoyar a la comunidad investigadora con recursos genéticos esenciales.
Principales métodos:
- Producción y construcción de bibliotecas.
- Secuenciación de alto rendimiento de ADN complementario (ADNc).
- Desarrollo de bases de datos y repositorios robustos para la gestión y el acceso a los datos.
- El avance de las tecnologías habilitadoras para la secuenciación y el análisis.
Principales resultados:
- Generación de una valiosa colección de clones de ADNc de mamíferos de cuerpo entero.
- Establecimiento de una base de datos de acceso público para estos recursos genéticos.
- Apoyo a diversas aplicaciones de investigación que requieren secuencias de genes de longitud completa.
Conclusiones:
- El proyecto de Colección de genes de mamíferos (MGC, por sus siglas en inglés) creó con éxito un recurso vital para la comunidad científica.
- La accesibilidad pública de estos recursos completos de ADNc acelera la investigación en genética de mamíferos y biología molecular.
- El proyecto destaca la importancia de los esfuerzos colaborativos a gran escala en genómica.
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