Video Experimental Relacionado
Updated: Jul 5, 2026
10:53
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
Published on: September 15, 2014
La excitación rápida evocada por las neurotrofinas a través de los receptores TrkB
K W Kafitz1, C R Rose, H Thoenen
1Institut für Physiologic, Technische Universität München, Germany.
Nature
|November 30, 1999
Resumen
Las neurotrofinas, como el factor neurotrófico derivado del cerebro (BDNF), excitan rápidamente las neuronas centrales mediante la activación de los canales de sodio. Esta rápida acción excitatoria posiciona las neurotrofinas como potentes neuro-excitantes endógenos en el cerebro de los mamíferos.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- Las neurotrofinas son proteínas cruciales que regulan la supervivencia neuronal, la diferenciación y la plasticidad.
- Su papel en la modulación de la plasticidad neuronal dependiente de la actividad está bien establecido.
- Un papel excitador directo en las neuronas centrales permaneció en gran parte inexplorado.
Objetivo del estudio:
- Para investigar la acción directa de las neurotropinas en las neuronas centrales.
- Para determinar si las neurotropinas pueden provocar potenciales de acción.
- Para identificar el mecanismo y la potencia de la excitación neuronal inducida por neurotrofinas.
Principales métodos:
- Grabaciones electrofisiológicas en las neuronas hipocampal, cortical y cerebelosa.
- Aplicación de varias neurotrofinas (BDNF, NT-4/5, NT-3, NGF) en diferentes concentraciones.
- Bloqueo farmacológico utilizando K-252a para investigar la participación de los canales iónicos.
Principales resultados:
- El factor neurotrófico derivado del cerebro (BDNF) y la neurotrofina-4/5 despolarizaron rápidamente las neuronas centrales, provocando potenciales de acción.
- Esta excitación se produjo en concentraciones significativamente más bajas que el glutamato.
- El efecto fue mediado por una conductancia de iones de sodio bloqueada por K-252a, lo que sugiere la participación del receptor Trk.
Conclusiones:
- Las neurotrofinas, particularmente el BDNF, poseen una acción excitadora potente y rápida en las neuronas centrales de los mamíferos.
- Esta rápida excitación neurotrófica está mediada por la activación del canal de sodio.
- Las neurotrofinas se identifican como neuro-excitantes endógenos significativos en el sistema nervioso central.
Más Videos Relacionados
13:46An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
Published on: July 11, 2020
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
Videos de Conceptos Relacionados
01:00Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
01:15Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
01:26Receptor Tyrosine Kinases
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
01:30Regulation of Food Intake
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...