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La célula endotelial vascular se adhiere al ensamblaje de unión y la morfogénesis inducida por el
M J Lee1, S Thangada, K P Claffey
1Center for Vascular Biology, Department of Physiology, University of Connecticut Health Center, Farmington 06030-3501, USA.
Cell
|November 11, 1999
Resumen
El 1-fosfato de esfingosina (SPP), un lípido, activa receptores específicos en las células endoteliales. Este proceso es crucial para la formación de nuevos vasos sanguíneos, un aspecto clave de la angiogénesis.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las células endoteliales vasculares forman redes capilares en respuesta a los factores angiogénicos.
- El 1-fosfato de esfingosina (SPP) es un lípido bioactivo derivado de las plaquetas.
Objetivo del estudio:
- Para investigar el papel de la esfingosina-1-fosfato (SPP) en la regulación de la angiogénesis.
- Identificar los receptores específicos y las vías de señalización involucradas en la angiogénesis mediada por SPP.
Principales métodos:
- Investigó la activación de los receptores EDG-1 y EDG-3 por SPP en las células endoteliales.
- Se analizaron las vías de señalización aguas abajo, incluyendo la proteína quinasa activada por Gi/mitógeno/supervivencia celular y el ensamblaje de unión de adherencias acopladas a Rho/Rac.
- Evaluó el requisito de la señalización EDG-1 y EDG-3 para la morfogénesis de las células endoteliales.
- Evaluó los efectos sinérgicos del SPP con los factores de crecimiento angiogénico polipéptido in vivo.
Principales resultados:
- SPP activa los receptores acoplados a proteínas G EDG-1 y EDG-3 en las células endoteliales.
- La SPP induce la vía de la proteína quinasa activada por Gi/mitógeno/supervivencia celular.
- SPP promueve pequeñas GTPasa Rho- y Rac-acoplado se adhiere ensamblaje de unión.
- Tanto las vías de señalización EDG-1 como EDG-3 son esenciales para la formación de redes de tipo capilar por parte de las células endoteliales.
- SPP mejora la formación de neovasos maduros in vivo cuando se combina con factores de crecimiento angiogénico.
Conclusiones:
- El 1-fosfato de esfingosina (SPP) es un nuevo regulador de la angiogénesis.
- La SPP actúa a través de los receptores EDG-1 y EDG-3 para controlar la morfogénesis de las células endoteliales y la formación de nuevos vasos sanguíneos.
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