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Regulación de la miosina fosfatasa por una interacción específica con la proteína quinasa Ialfa Ialfa dependiente del

H K Surks¹, N Mochizuki, Y Kasai

¹Molecular Cardiology Research Institute and Cardiology Division, Department of Medicine, Tufts University School of Medicine and New England Medical Center, Boston, MA 02111, USA.

Science (New York, N.Y.)

|November 24, 1999

Resumen

La proteína quinasa Ialfa dependiente del monofosfato de guanosina cíclica (cGKIalpha) se dirige al aparato contráctil del músculo liso a través de la interacción de la subunidad de unión a la miosina (MBS). Esta interacción es crucial para regular el tono muscular liso vascular mediante el control de la desfosforilación de la cadena ligera de miosina.

Área de la Ciencia:

Biología molecular La biología molecular.
Fisiología celular La fisiología celular.
La bioquímica es la bioquímica.

Sus antecedentes:

La contracción / relajación del músculo liso está controlada por la miosina, la cinasa de cadena ligera y la fosfatasa.
La proteína quinasa Ialfa dependiente del monofosfato cíclico de guanosina (cGMP) (cGKIalpha) es clave en la relajación del músculo liso vascular.
El óxido nítrico (NO) y las vías cGMP regulan el tono vascular.

Objetivo del estudio:

Para dilucidar el mecanismo por el cual cGKIalpha regula el tono del músculo liso vascular.
Para identificar la interacción específica que media la función de cGKIalpha en el aparato contráctil.
Para demostrar la necesidad de esta interacción para la relajación fisiológica del músculo liso.

Principales métodos:

Investigó la interacción entre cGKIalpha y las subunidades de la miosina fosfatasa.
Se utilizaron ensayos bioquímicos para confirmar la unión por medio de la cremallera de la leucina.
Examinó las consecuencias funcionales de interrumpir la interacción cGKIalpha-MBS en la fosforilación de la cadena ligera de miosina.

Principales resultados:

cGKIalpha se une directamente a la subunidad de unión a la miosina (MBS) de la miosina fosfatasa a través de un motivo de cremallera de leucina.
Esta interacción apunta con éxito cGKIalpha al aparato contráctil del músculo liso.
La interrupción de la interacción cGKIalpha-MBS abolió la desfosforilación dependiente de cGMP de la cadena ligera de miosina.

Conclusiones:

La interacción entre cGKIalpha y MBS es esencial para dirigir la quinasa a su sitio de acción.
Esta interacción específica es crítica para la regulación fisiológica del tono muscular liso vascular.
La comprensión de este mecanismo proporciona información sobre la señalización NO / cGMP en el músculo liso.

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