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Las membranas de Golgi son absorbidas y reemergen de la ER durante la mitosis
K J Zaal1, C L Smith, R S Polishchuk
1Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Istitutes of Health, Bethesda, Maryland 20892-5430, USA.
Cell
|December 28, 1999
Resumen
El aparato de Golgi se dispersa en la ER durante la mitosis y se reforma a través de las vías de reciclaje de la ER, desafiando los modelos anteriores de herencia y génesis de Golgi.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Dinámica de las Organetas Dinámica de las Organetas
Sus antecedentes:
- El aparato de Golgi es crucial para la modificación y el transporte de proteínas.
- Su comportamiento durante la división celular (mitosis) ha sido objeto de debate.
- Comprender la dinámica de Golgi es clave para la división celular y la herencia.
Objetivo del estudio:
- Para investigar el reciclaje de proteínas de Golgi en las células de interfase.
- Para monitorear los cambios en la estructura de Golgi durante la mitosis.
- Para dilucidar el mecanismo de la reformación de Golgi después de la mitosis.
Principales métodos:
- Imágenes cuantitativas y foto blanqueamiento en células vivas.
- Seguimiento de las proteínas Golgi etiquetadas con GFP.
- Microscopía inmunoelectrónica (inmuno-EM), también conocida como microscopía inmunológica.
Principales resultados:
- Las proteínas de Golgi se reciclan cada 1,5 horas en la interfase.
- Durante la mitosis, las proteínas de Golgi se redistribuyen en el ER.
- No se detectaron vesículas mitóticas de Golgi distintas; los componentes se fusionan con el ER.
- La reformación de Golgi después de la mitosis requiere la exportación de ER.
Conclusiones:
- El Golgi se dispersa en el ER durante la mitosis.
- La reforma de Golgi se basa en las rutas de exportación y reciclaje de ER.
- Esto define un nuevo paradigma para la biogénesis y la herencia de Golgi.
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