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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
Published on: May 17, 2016
El lenguaje de las modificaciones covalentes de la histona
1Department of Biochemistry and Molecular Genetics, University of Virginia Health Science Center, Charlottesville 22908, USA.
Nature
|January 19, 2000
Resumen
Las modificaciones de las histonas en las proteínas de envasado del ADN pueden formar un "código de histona". Este código, leído por otras proteínas, probablemente regula procesos celulares cruciales.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las proteínas histonas y los nucleosomas son fundamentales para la estructura de la cromatina eucariota.
- Las modificaciones post-traduccionales en las colas de histona se conservan, pero sus funciones son en gran parte desconocidas.
Objetivo del estudio:
- Proponer un modelo para la función de las modificaciones histónicas.
- Introducir el concepto de un "código de historia".
Principales métodos:
- Revisión de las pruebas bioquímicas y genéticas existentes.
- Desarrollo del marco teórico. desarrollo.
Principales resultados:
- Las modificaciones de las histonas, que actúan solas o juntas, sugieren un mecanismo regulador.
- Estas modificaciones pueden funcionar secuencialmente o combinatoriamente.
Conclusiones:
- Se propone una hipótesis de "código histónico".
- Este código es interpretado por otras proteínas para impulsar eventos celulares aguas abajo.
- Las modificaciones de la histona juegan un papel clave en los procesos basados en la cromatina.
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