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La quinasa del receptor S determina la autoincompatibilidad en el estigma de Brassica

T Takasaki¹, K Hatakeyama, G Suzuki

¹Research Institute of Seed Production Co., Ltd., Sendai, Japan. taka@kobe-u.ac.jp

|March 8, 2000

Resumen

La autoincompatibilidad en Brassica se basa en los genes de la S receptor quinasa (SRK) y la glicoproteína del locus S (SLG). SRK por sí solo determina la especificidad del rechazo del polen, mientras que SLG mejora la respuesta de autoincompatibilidad.

Área de la Ciencia:

Biología reproductiva de las plantas Biología reproductiva de las plantas
Genética molecular genética molecular.
Mejoramiento de plantas Mejoramiento de plantas.

Sus antecedentes:

La autoincompatibilidad (SI) es una barrera reproductiva crucial en Brassica, que impide la autofertilización.
El locus S, que controla el SI, involucra dos genes clave: la quinasa del receptor S (SRK) y la glicoproteína del locus S (SLG).
Estos genes se expresan en las células papillares del estigma y se cree que median el reconocimiento y el rechazo del polen.

Objetivo del estudio:

Para aclarar los roles individuales de SRK y SLG en la autoincompatibilidad de Brassica.
Para determinar si SRK o SLG por sí solos pueden conferir el rechazo del auto-pollen.
Investigar el efecto sinérgico de SRK y SLG en la respuesta de autoincompatibilidad.

Principales métodos:

Transformación genética de plantas autoincompatibles de Brassica rapa con transgenes SRK28 y SLG28.
Análisis del rechazo del polen en plantas transgénicas que expresan SRK28 solo, SLG28 solo, o ambos.
Evaluación fenotípica de la respuesta de autoincompatibilidad en plantas Brassica modificadas.

Principales resultados:

La expresión del transgen SRK28 por sí sola confería la capacidad de rechazar el polen propio (S28).
La expresión transgénica de SLG28 por sí sola no confería rechazo del auto-pollen.
La capacidad de rechazo de polen mediada por SRK28 fue significativamente mejorada por la coexpresión de SLG28.

Conclusiones:

SRK por sí solo es suficiente para determinar la especificidad del haplotipo S del estigma en la autoincompatibilidad de Brassica.
El SLG funciona como un modulador, mejorando la manifestación completa de la respuesta de autoincompatibilidad.
Estos hallazgos aclaran las funciones distintas pero cooperativas de SRK y SLG en el aislamiento reproductivo de Brassica.

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