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Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging
Published on: April 29, 2016
Receptores gustativos candidatos en las Drosophila
P J Clyne1, C G Warr, J R Carlson
1Department of Molecular, Cellular, and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520-8103, USA.
Science (New York, N.Y.)
|March 10, 2000
Resumen
Los investigadores identificaron una gran familia de siete proteínas de dominio transmembrana en Drosophila, con 18 genes expresados en el labelo, lo que sugiere que pueden funcionar como receptores del gusto para la percepción gustativa.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La neurociencia es la neurociencia.
- Genética La genética.
Sus antecedentes:
- Los mecanismos moleculares de la percepción del gusto y los eventos iniciales de señalización del gusto en los animales siguen siendo en gran medida desconocidos.
- Siete proteínas de dominio transmembrana representan una diversa familia de proteínas con funciones conocidas en la percepción sensorial.
Objetivo del estudio:
- Para identificar posibles nuevos receptores gustativos en Drosophila.
- Para investigar los patrones de expresión de una familia de proteínas recientemente identificada en los órganos gustativos.
Principales métodos:
- Análisis bioinformático del genoma de Drosophila para identificar siete proteínas de dominio transmembrana.
- Análisis de la expresión génica utilizando PCR cuantitativa y hibridación in situ.
- Examen de la expresión génica en un mutante de Drosophila que carece de neuronas gustativas (pox-neuro70).
Principales resultados:
- Se identificó una familia grande y diversa de 19 proteínas de siete dominios transmembrana.
- Dieciocho de estos 19 genes mostraron expresión específica en el Drosophila labellum, un órgano gustativo primario.
- La expresión de estos genes estaba ausente en otros tejidos y en el labelo de la varicela-neuro70 mutante.
Conclusiones:
- La familia de proteínas identificada, basada en la similitud estructural y la expresión específica del tejido en los órganos gustativos, probablemente codifica una familia divergente de receptores del gusto.
- Estos hallazgos proporcionan una base para la comprensión de la base molecular de la percepción del gusto en Drosophila.
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