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Un progenitor mieloide común clonogénico que da lugar a todos los linajes mieloides
K Akashi1, D Traver, T Miyamoto
1Department of Pathology, Stanford University School of Medicine, California 94305, USA. akashi@leland.stanford.edu
Nature
|March 21, 2000
Resumen
Los investigadores identificaron células progenitoras tempranas en el desarrollo de la sangre. Los progenitores mieloides comunes dan lugar a todos los linajes mieloides, marcando un punto clave de ramificación en la hematopoyesis.
Área de la Ciencia:
- Hematología Hematología.
- Biología del desarrollo Biología del desarrollo.
- Biología de las células madre Biología de las células madre
Sus antecedentes:
- Las células madre hematopoyéticas (HSC) se diferencian en varios tipos de células sanguíneas, perdiendo progresivamente la auto-renovación y el potencial de linaje.
- Los puntos de compromiso de linaje precisos para los progenitores derivados de HSC siguen siendo en gran medida indefinidos.
- Trabajos anteriores identificaron un progenitor linfoide común (CLP) para las células T, B y NK.
Objetivo del estudio:
- Identificar y caracterizar la población progenitora mieloide más antigua.
- Para delinear los puntos de ramificación en la hematopoyesis temprana.
- Proporcionar un perfil inicial de expresión génica de poblaciones progenitoras purificadas.
Principales métodos:
- Identificación y purificación prospectiva de células progenitoras utilizando marcadores de superficie celular y citometría de flujo.
- Análisis funcionales para evaluar el potencial de diferenciación de progenitores purificados.
- Perfilado de la expresión génica de los genes relacionados con la hematopoyesis.
Principales resultados:
- Se identificó y purificó un progenitor mieloide común (CMP) clonogénico.
- Las CMP dan lugar a todos los linajes mieloides, incluidos los progenitores de megacariocitos/eritrocitos y granulocitos/macrófagos.
- CLP y CMP representan los primeros puntos de bifurcación del linaje linfoide y mieloide.
Conclusiones:
- La población CMP es complementaria a la CLP, representando el más antiguo progenitor mieloide.
- La diferenciación de CMP en linajes de megakariocitos/eritrocitos o granulocitos/macrófagos son eventos que se excluyen mutuamente.
- Este estudio proporciona un marco para comprender el compromiso temprano del linaje en la hematopoyesis.
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