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Las lipoproteínas oxidadas degradan la capa superficial endotelial: implicaciones para la adhesión de las células
H Vink1, A A Constantinescu, J A Spaan
1Department of Medical Physics, University of Amsterdam, Amsterdam, The Netherlands. h.vink@amc.uva.nl
Circulation
|April 4, 2000
Resumen
Las lipoproteínas oxidadas de baja densidad (Ox-LDL) interrumpen la capa superficial endotelial, aumentando la adhesividad de la pared vascular. Este efecto está mediado por los radicales libres derivados del oxígeno y puede ser bloqueado por los antioxidantes.
Área de la Ciencia:
- Biología Cardiovascular Biología Cardiovascular
- La función endotelial es la función endotelial.
- Investigación de la aterosclerosis Investigación de la aterosclerosis.
Sus antecedentes:
- La capa de superficie endotelial (ESL) mantiene una separación entre las células sanguíneas que fluyen y la superficie de la célula endotelial (CE).
- Las lipoproteínas oxidadas de baja densidad (Ox-LDL) están implicadas en la aterogénesis.
- El papel de Ox-LDL en la disrupción de la ESL y la adherencia vascular requiere una aclaración.
Objetivo del estudio:
- Investigar la hipótesis de que el Ox-LDL interrumpe el ESL, lo que lleva a un aumento de la adhesividad de la pared vascular.
- Determinar el papel de los radicales libres derivados del oxígeno en los cambios en la ESL inducidos por Ox-LDL.
Principales métodos:
- Se utilizó microscopía intravital para observar la distancia de la superficie de los eritrocitos-CE en el músculo del hámster cremaster.
- Ox-LDL fue preparado por CuSO ((4) oxidación de LDL nativo.
- Se evaluaron los efectos de Ox-LDL y antioxidantes (superoxide dismutasa y catalasa) en la dimensión de la ESL y la adhesión plaqueta-CE.
Principales resultados:
- Ox-LDL disminuyó transitoriamente la dimensión de la ESL en un 60% en 25 minutos.
- Esta reducción de la ESL se correlacionó con un aumento transitorio de las adherencias plaquetas-CE.
- La administración combinada de superóxido dismutasa y catalasa bloqueó completamente los efectos de Ox-LDL en la ESL y la adhesión plaquetaria.
Conclusiones:
- Los radicales libres derivados del oxígeno median la ruptura de la capa superficial endotelial por Ox-LDL.
- La alteración de la ESL por Ox-LDL contribuye al aumento de la adhesividad de la pared vascular.
- El tratamiento con antioxidantes puede prevenir los cambios en la ESL inducidos por Ox-LDL y la adhesión plaquetaria asociada.
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