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Los receptores de estrógeno de la superficie celular median la liberación de óxido nítrico dependiente del calcio en
G B Stefano1, V Prevot, J C Beauvillain
1Neuroscience Research Institute, State University of New York (SUNY) at Old Westbury, NY 11568-0210, USA.
Circulation
|April 4, 2000
Resumen
El estrógeno aumenta rápidamente la liberación de óxido nítrico (NO) de las células endoteliales humanas. Esto ocurre a través de un receptor de estrógeno en la superficie celular, activando el calcio intracelular, y puede explicar el estrógeno.
Área de la Ciencia:
- Endocrinología Endocrinología.
- Fisiología cardiovascular fisiología cardiovascular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La terapia de reemplazo de estrógenos puede reducir los eventos cardiovasculares en mujeres posmenopáusicas.
- El mecanismo preciso detrás de los beneficios cardiovasculares de los estrógenos no se entiende completamente.
- El óxido nítrico (NO) es un factor derivado del endotelio clave que protege los vasos sanguíneos.
Objetivo del estudio:
- Para investigar los efectos agudos de los niveles de estrógeno fisiológico en la liberación de NO de las células endoteliales humanas.
- Para determinar si el estrógeno estimula la liberación de NO a través de un receptor de la superficie celular.
- Para dilucidar la vía de señalización involucrada en la liberación de NO mediada por estrógenos.
Principales métodos:
- Medición en tiempo real de la liberación de NO utilizando una sonda amperométrica.
- Exposición de la arteria torácica interna humana y las células endoteliales arteriales al 17beta-estradiol.
- Utilizando el 17beta-estradiol conjugado con la albúmina sérica bovina para evaluar la participación de los receptores.
- El uso de tamoxifeno para inhibir los receptores de estrógeno.
- Medición de los transitorios de calcio intracelulares con microfluorometría de doble emisión.
Principales resultados:
- El 17beta-estradiol estimuló rápidamente la liberación de NO de las células endoteliales de una manera dependiente de la concentración.
- La evidencia sugiere que el estrógeno actúa a través de un receptor de la superficie celular, como lo indica el antagonismo conjugado del estradiol y el tamoxifeno.
- La liberación de NO estimulada por el estrógeno depende del aumento inicial del calcio intracelular.
Conclusiones:
- Las dosis fisiológicas de estrógeno estimulan agudamente la liberación de óxido nítrico (NO) de las células endoteliales humanas.
- Esta estimulación está mediada por un receptor de estrógeno de la superficie celular.
- La vía implica la activación de la señalización del calcio intracelular.
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