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Un cotransportador electroneutral de sodio/bicarbonato NBCn1 y el canal de sodio asociado

I Choi¹, C Aalkjaer, E L Boulpaep

¹Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

|June 13, 2000

Resumen

Los investigadores identificaron nuevas variantes electroneutrales del cotransportador de bicarbonato de sodio (NBCn1) que son cruciales para la regulación del pH intracelular. Estos transportadores son dependientes del sodio y el bicarbonato, pero no dependientes del cloruro en las células de mamíferos.

Área de la Ciencia:

Fisiología Fisiología Fisiología.
Biología Molecular Biología Molecular
La bioquímica es la bioquímica.

Sus antecedentes:

La regulación del pH intracelular es vital para la función celular.
Los transportadores de HCO3- impulsados por Na+ neutros en electrones, incluidos los intercambiadores de Cl-/HCO3- impulsados por Na+ y los cotransportadores de Na+/HCO3, juegan un papel clave.
Distinguir la dependencia de Cl de estos transportadores en las células de mamíferos es un desafío.

Objetivo del estudio:

Clonar y caracterizar las variantes de un cotransportador electroneutral Na+/HCO3- (NBCn1) del músculo liso de la rata.
Determinar las propiedades funcionales, incluida la dependencia iónica, de las variantes NBCn1 clonadas.
Para investigar el potencial de la conductancia Na+ asociada con la expresión de NBCn1.

Principales métodos:

Clonado de tres variantes de NBCn1 a partir del músculo liso de la rata.
Expresión de la variante NBCn1-B en los ovocitos de Xenopus.
Caracterización funcional de NBCn1-B, evaluando la dependencia de Na+, HCO3 y Cl.
Análisis de la conductancia de Na+ utilizando electrofisiología y un inhibidor específico (4,4-diisothiocyanatostilbeno-2,2'-disulfonato).

Principales resultados:

Se clonaron tres variantes de NBCn1, mostrando una alta identidad con los clones de músculo esquelético humano y una identidad más baja con los NBC electrogénicos y los intercambiadores de aniones.
Se encontró que el NBCn1-B expresado es electroneutral, dependiente de Na+ y HCO3-, pero independiente de Cl-.
La expresión de bajo nivel de NBCn1-B en los ovocitos indujo una conductividad de Na+ estimulada lenta e irreversiblemente.

Conclusiones:

NBCn1 representa una clase distinta de cotransportadores electroneutrales Na+/HCO3.
NBCn1 juega un papel importante en la homeostasis del pH celular independiente del cloruro.
NBCn1 puede contribuir a la excitabilidad celular a través de la conductancia Na+ asociada.

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