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Fluorescence Activated Cell Sorting of Plant Protoplasts
Published on: February 18, 2010
La señalización celular y el movimiento celular por los factores de transcripción florales LEAFY y APETALA1
A Sessions1, M F Yanofsky, D Weigel
1Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Science (New York, N.Y.)
|August 5, 2000
Resumen
La proteína LEAFY (LFY) se mueve entre las células para controlar el desarrollo de la flor de Arabidopsis, mientras que APETALA1 (AP1) actúa dentro de su propia célula. Esto revela distintos roles de señalización célula-célula para estos reguladores florales clave.
Área de la Ciencia:
- Biología vegetal Biología vegetal
- Biología del desarrollo Biología del desarrollo.
- Genética molecular genética molecular.
Sus antecedentes:
- LEAFY (LFY) y APETALA1 (AP1) son factores de transcripción cruciales que regulan el desarrollo de la flor de Arabidopsis.
- Ellos activan conjuntos superpuestos de genes homeóticos, controlando la identidad del órgano floral.
Objetivo del estudio:
- Para investigar las capacidades de señalización célula-célula de LFY y AP1 dentro del meristema floral.
- Para determinar si estos factores de transcripción se mueven entre las células o actúan de forma autónoma.
Principales métodos:
- Utilizamos el análisis sectorial en Arabidopsis para estudiar la función genética.
- Expresión dirigida empleada en plantas transgénicas para observar la localización y actividad de proteínas.
- Analizó la activación de genes objetivo homeóticos.
Principales resultados:
- La proteína LFY demostró una acción de largo alcance y se trasladó a las células adyacentes, activando directamente los genes diana.
- AP1 exhibió principalmente actividad celular autónoma con efectos no autónomos limitados.
- Los efectos no autónomos de AP1 parecían estar mediados a través de genes aguas abajo.
Conclusiones:
- La LFY funciona a través del movimiento y la señalización celular, influyendo en el desarrollo floral a través de distancias.
- AP1 actúa predominantemente dentro de la célula donde se expresa, con distintos mecanismos de regulación.
- Estos hallazgos resaltan las estrategias de comunicación celular diferencial empleadas por los reguladores florales clave.
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