Video Experimental Relacionado
Updated: Jul 8, 2026
08:33
An Ex vivo Culture System to Study Thyroid Development
Published on: June 6, 2014
La hormona tiroidea coordina la maduración del control respiratorio y la expresión del translocador de nucleótidos de
1Division of Cardiology, Department of Pediatrics, University of Washington School of Medicine, and Children's Hospital and Regional Medical Center, Seattle, WA 98105-0371, USA. mportm@chmc.org
Circulation
|September 12, 2000
Resumen
La hormona tiroidea regula la maduración del corazón mediante el aumento de los niveles de translocadores de nucleótidos de adenina mitocondrial (ANT). Este cambio reduce el control respiratorio mediado por ADP durante el desarrollo postnatal en corderos.
Área de la Ciencia:
- Fisiología cardiovascular fisiología cardiovascular.
- Biología mitocondrial Biología mitocondrial
- Endocrinología Endocrinología.
Sus antecedentes:
- El metabolismo energético del corazón sufre cambios significativos durante el desarrollo posnatal.
- La acumulación de translocadores de nucleótidos de adenina mitocondrial (ANT) está relacionada con el control respiratorio.
- Se supone que la hormona tiroidea regula estos cambios en el desarrollo.
Objetivo del estudio:
- Investigar el papel de la hormona tiroidea en la regulación de la maduración postnatal del metabolismo energético cardíaco.
- Para determinar si la hormona tiroidea influye en la expresión de ANT y su impacto en el control respiratorio.
Principales métodos:
- (31) Se utilizó la espectroscopia MR para evaluar los fosfatos de alta energía del miocardio y el consumo de oxígeno en corderos tiroidectomizados y de control.
- Se emplearon las mediciones Western y Northern para cuantificar los niveles de proteínas y ARNm de la ANT y la beta-F(1)-ATPasa.
Principales resultados:
- Los corderos tiroidectomizados exhibieron un menor consumo máximo de oxígeno y un deterioro en el mantenimiento de fosfato de alta energía en comparación con los controles.
- Se observaron niveles aumentados de ADP citosólico en corderos tiroidectomizados.
- Los corazones de control mostraron niveles significativamente más altos de ANT mRNA (2 veces) y proteínas (4 veces) en comparación con los corderos tiroidectomizados.
Conclusiones:
- La hormona tiroidea es un regulador clave del cambio de maduración lejos del control respiratorio mediado por ADP en el corazón.
- La hormona tiroidea influye en la expresión de ANT a un nivel pretranslacional, indicado por el aumento de los niveles de ARNm y proteínas de ANT.
Videos de Conceptos Relacionados
01:27Transducer Mechanism: Enzyme-Linked Receptors
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
01:23The Thyroid Gland
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
01:18Functions of Thyroid Hormones
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
01:11Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
01:27Hyperthyroidism II: Pathophysiology
Hyperthyroidism is a hypermetabolic state caused by elevated levels of thyroid hormones, triiodothyronine (T3) and thyroxine (T4). It results from dysregulation at the thyroid, pituitary, or immune system level and affects multiple organ systems.PathophysiologyThe most common cause of hyperthyroidism is Graves’ disease, an autoimmune disorder in which antibodies, specifically thyroid-stimulating antibodies (TSAb), a subtype of TSH receptor antibodies (TRAb), bind to and activate TSH receptors...