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La PAC define una segunda vía de señalización requerida para el transporte de glucosa estimulado por insulina
C A Baumann1, V Ribon, M Kanzaki
1Department of Physiology, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA.
Nature
|September 23, 2000
Resumen
La insulina es una insulina.
Área de la Ciencia:
- Biología celular Biología celular.
- Los mecanismos moleculares de la señalización de la insulina.
Sus antecedentes:
- La insulina regula el transporte de glucosa en las células grasas y musculares.
- Los mecanismos moleculares precisos de la acción de la insulina no se comprenden completamente.
- La activación del receptor de insulina inicia las cascadas de señalización intracelular.
Objetivo del estudio:
- Aclarar los mecanismos moleculares subyacentes al transporte de glucosa estimulado por insulina.
- Para identificar las proteínas involucradas en la vía de señalización del receptor de insulina.
- Comprender el papel de las proteínas Cbl y CAP en la acción de la insulina.
Principales métodos:
- Cribado de dos híbridos de levadura para identificar las interacciones de proteínas.
- Ensayos bioquímicos para estudiar la formación y localización de complejos proteicos.
- Experimentos celulares que utilizan adipocitos 3T3-L1 para evaluar la absorción de glucosa.
Principales resultados:
- Identificó flotillin como un socio vinculante para el complejo CAP-Cbl.
- Se demostró que la flotillina dirige el complejo CAP-Cbl a las balsas de lípidos.
- Se demostró que la localización del complejo CAP-Cbl a las balsas lipídicas es esencial para la absorción de glucosa estimulada por insulina.
- Se descubrió que el bloqueo de la localización de la CAP inhibe el efecto de la insulina en el transporte de glucosa sin afectar la señalización de la fosfatidilinositol-3-OH quinasa.
Conclusiones:
- El complejo Cbl-CAP, localizado en las balsas lipídicas a través de la flotillina, es un componente crítico del transporte de glucosa regulado por la insulina.
- Esta vía representa un mecanismo clave para controlar la absorción de glucosa en respuesta a la insulina.
- Dirigirse a esta vía podría ofrecer nuevas estrategias para controlar el metabolismo de la glucosa.
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