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El papel global de las enzimas remodeladoras de la cromatina en la expresión génica mitótica
J E Krebs1, C J Fry, M L Samuels
1Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605, USA.
Cell
|September 28, 2000
Resumen
Las enzimas remodeladoras de cromatina dependientes de ATP (SWI/SNF) controlan el reclutamiento de la histona acetiltransferasa (Gcn5p) para la expresión génica durante la mitosis. Este proceso es esencial para la salida mitótica y la regulación génica en la cromatina mitótica condensada.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Biología celular Biología celular.
Sus antecedentes:
- La expresión génica de los eucariotas está regulada por enzimas remodeladoras de cromatina dependientes de ATP como SWI/SNF.
- Las histonas acetiltransferasas (HAT), como Gcn5p, son cruciales para la expresión génica.
- La interacción entre SWI/SNF y Gcn5p en la regulación de la expresión génica, particularmente durante la mitosis, no se entiende completamente.
Objetivo del estudio:
- Investigar el papel de SWI/SNF en el reclutamiento de la actividad Gcn5p HAT a los genes durante la mitosis tardía.
- Determinar la participación de SWI/SNF y Gcn5p en la regulación de la salida mitótica.
- Explorar la dependencia de la expresión génica en SWI/SNF y Gcn5p en diferentes fases del ciclo celular.
Principales métodos:
- Análisis de la reclutamiento de la actividad Gcn5p HAT a varios genes.
- Evaluación del papel de SWI/SNF en el reclutamiento de Gcn5p durante la mitosis tardía.
- Examen de cambios en la expresión génica en las vías dependientes de SWI/SNF y Gcn5p.
Principales resultados:
- La remodelación SWI/SNF controla el reclutamiento de Gcn5p HAT a muchos genes en la mitosis tardía.
- SWI / SNF y Gcn5p están involucrados en la regulación de la salida mitótica.
- La expresión génica de interfase (GAL1, HIS3, PHO5, PHO8) es independiente de SWI / SNF para el reclutamiento de Gcn5p, pero se vuelve dependiente cuando las células se detienen en la mitosis tardía.
Conclusiones:
- SWI/SNF juega un papel crítico en el reclutamiento de la actividad Gcn5p HAT a los genes durante la mitosis.
- Tanto SWI/SNF como Gcn5p son esenciales para regular la salida mitótica y la expresión génica en la cromatina mitótica condensada.
- La dependencia de SWI / SNF para el reclutamiento y la expresión génica de Gcn5p es específica del ciclo celular, destacando el impacto de la condensación de la cromatina.
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