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La especificidad de la vía Ras está determinada por la integración de múltiples factores de transcripción activados
M S Halfon1, A Carmena, S Gisselbrecht
1Department of Medicine, Brigham and Women's Hospital, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA.
Cell
|October 29, 2000
Resumen
La señalización Wingless (Wg) y Decapentaplegic (Dpp) especifican el desarrollo del músculo y el progenitor del corazón de Drosophila actuando en paralelo a Ras. Estas vías integran múltiples factores de transcripción para controlar la especificidad de la señalización Ras.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización molecular.
- Genética La genética.
Sus antecedentes:
- Las vías de señalización Ras regulan diversas salidas celulares, pero los mecanismos que confieren especificidad no se comprenden completamente.
- La señalización mediada por la receptor tirosina quinasa (RTK) es crucial para la determinación del destino celular en el desarrollo.
Objetivo del estudio:
- Investigar cómo las vías de señalización Wingless (Wg) y Decapentaplegic (Dpp) contribuyen a la especificidad de señalización Ras en el desarrollo del músculo de Drosophila y el progenitor cardíaco.
- Para dilucidar los mecanismos transcripcionales combinatorios subyacentes a la señalización inductiva en estas células progenitoras.
Principales métodos:
- Análisis de los patrones de expresión génica en embriones de Drosophila.
- Investigando la interacción entre las vías Wg, Dpp y Ras/MAPK.
- Estudiar la función de los factores de transcripción específicos (dTCF, Mad, Pointed, Twist, Tinman) en el desarrollo de los progenitores.
Principales resultados:
- Wg y Dpp confieren competencia para la inducción mediada por RTK de músculo específico de Drosophila y progenitores cardíacos.
- Wg y Dpp actúan tanto aguas arriba como en paralelo a la señalización Ras.
- Estas vías coordinan los efectos directos de múltiples factores de transcripción en un potenciador de genes de identidad de progenitor.
- La integración de Pointed con dTCF, Mad, Twist y Tinman determina la especificidad de señalización Ras.
Conclusiones:
- La señalización Wg y Dpp son reguladores críticos de la especificidad de la señalización Ras durante el desarrollo del músculo y el corazón de Drosophila.
- Un código combinatorio de factores de transcripción integra señales para controlar con precisión la identidad y diferenciación de las células progenitoras.
- La comprensión de estos mecanismos moleculares proporciona información sobre las redes de señalización del desarrollo.
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