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Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
La nucleofosmina/B23 es un objetivo de CDK2/ciclina E en la duplicación del centrosoma
M Okuda1, H F Horn, P Tarapore
1Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Ohio 45267, USA.
Cell
|October 29, 2000
Resumen
La nucleofosmina (NPM/B23) es crucial para la duplicación del centrosoma en las células animales. La fosforilación mediada por CDK2 / ciclina E de NPM / B23 inicia este proceso vital del ciclo celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La duplicación del centrosoma y el ADN son eventos coordinados del ciclo celular en las células animales.
- Se cree que la activación de CDK2 / ciclina E vincula el inicio de la replicación del ADN y la duplicación del centrosoma.
Objetivo del estudio:
- Para identificar sustratos de CDK2 / ciclina E involucrados en la duplicación del centrosoma.
- Para aclarar el papel de la nucleofosmina (NPM/B23) en el inicio de la duplicación del centrosoma.
Principales métodos:
- Identificación de NPM/B23 como un sustrato de CDK2 / ciclina E. Identificación de NPM/B23 como un sustrato de CDK2 / ciclina E. Identificación de NPM/B23 como un sustrato de CDK2 / ciclina E. Identificación de NPM/B23 como un sustrato de CDK2 / ciclina E.
- Ensayos inmunológicos utilizando anticuerpos anti-NPM/B23.
- Estudios in vivo de la iniciación de la duplicación del centrosoma.
- La expresión de los mutantes NPM/B23 no fosforilables.
Principales resultados:
- NPM/B23 se asocia específicamente con centrosomas no duplicados.
- La fosforilación mediada por CDK2 / ciclina E causa la disociación de NPM / B23 de los centrosomas.
- La inhibición de la fosforilación de NPM/B23 por anticuerpos suprime la duplicación del centrosoma.
- La expresión de NPM/B23 no fosforilable bloquea la duplicación del centrosoma.
Conclusiones:
- NPM / B23 es un objetivo clave de CDK2 / ciclina E para iniciar la duplicación del centrosoma.
- La fosforilación de NPM / B23 por CDK2 / ciclina E es esencial para la duplicación del centrosoma.
- NPM/B23 actúa como un enlace molecular entre la actividad de CDK2/ciclina E y la duplicación del centrosoma.
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