Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

[Clinical study on early loading restoration of superhydrophilic implants].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2021
Same author

Some Like It Sweet: Dendritic Cells Add Sugar to Their T(ea).

Cell·2020
Same author

Parametrically Tunable Soliton-Induced Resonant Radiation by Three-Wave Mixing.

Physical review letters·2017
Same author

Inhibitory effects of different substituted transition metal-based krebs-type sandwich structures on human hepatocellular carcinoma cells.

Dalton transactions (Cambridge, England : 2003)·2017
Same author

Design and performance of an ultra-high vacuum scanning tunneling microscope operating at dilution refrigerator temperatures and high magnetic fields.

The Review of scientific instruments·2013
Same author

Ultrafast and octave-spanning optical nonlinearities from strongly phase-mismatched quadratic interactions.

Physical review letters·2012

Video Experimental Relacionado

Updated: Jul 8, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

La respuesta al daño del ADN: poner los puntos de control en perspectiva.

B B Zhou1, S J Elledge

  • 1Department of Oncology Research, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA.

Nature
|December 2, 2000
PubMed
Resumen
Este resumen es generado por máquina.

La reparación adecuada del ADN es crucial para los mamíferos para prevenir trastornos y cáncer. Los organismos activan una vía compleja de respuesta al daño para manejar el daño cromosómico, controlar la detención del ciclo celular, la apoptosis y las redes de reparación del ADN.

Más Videos Relacionados

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Videos de Experimentos Relacionados

Last Updated: Jul 8, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Área de la Ciencia:

  • Genética La genética.
  • Biología Molecular Biología Molecular
  • Biología celular Biología celular.

Sus antecedentes:

  • La acumulación de daño en el ADN en los mamíferos está relacionada con trastornos y un mayor riesgo de cáncer.
  • Los organismos poseen intrincadas vías de respuesta al daño del ADN (DDR) para abordar los insultos cromosómicos.
  • Se sabe que la vía DDR regula la detención del ciclo celular y la apoptosis (muerte celular programada).

Objetivo del estudio:

  • Para dilucidar las funciones multifacéticas de la vía de respuesta al daño del ADN.
  • Investigar la regulación de las redes de reparación del ADN por la vía DDR.
  • Comprender las implicaciones de la reparación alterada del ADN en la salud de los mamíferos.

Principales métodos:

  • Este estudio se centra en los mecanismos reguladores de la vía de respuesta al daño del ADN.
  • Utilizando técnicas de biología molecular establecidas para analizar las respuestas celulares al daño del ADN.
  • Investigando la interacción entre el control del ciclo celular, la apoptosis y la activación de la reparación del ADN.

Principales resultados:

  • La vía de respuesta al daño del ADN es un regulador central de la homeostasis celular después del estrés genotóxico.
  • Más allá de la detención del ciclo celular y la apoptosis, la vía modula directamente los procesos de reparación del ADN.
  • La reparación disfuncional del ADN, orquestada por esta vía, contribuye a la patogénesis de la enfermedad.

Conclusiones:

  • La vía de respuesta al daño del ADN es un regulador crítico de la estabilidad genómica en los mamíferos.
  • Comprender esta vía ofrece información sobre la prevención y el tratamiento de trastornos y cánceres relacionados con el daño al ADN.
  • Se justifica una mayor investigación sobre la activación directa de las redes de reparación del ADN por la vía DDR.